Structure of the ArgRS-GlnRS-AIMP1 complex and its implications for mammalian translation
DC Field | Value | Language |
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dc.contributor.author | Fu, Yaoyao | - |
dc.contributor.author | Kim, Youngran | - |
dc.contributor.author | Jin, Kyeong Sik | - |
dc.contributor.author | Kim, Hyun Sook | - |
dc.contributor.author | Kim, Jong Hyun | - |
dc.contributor.author | Wang, DongMing | - |
dc.contributor.author | Park, Minyoung | - |
dc.contributor.author | Jo, Chang Hwa | - |
dc.contributor.author | Kwon, Nam Hoon | - |
dc.contributor.author | Kim, Doyeun | - |
dc.contributor.author | Kim, Myung Hee | - |
dc.contributor.author | Jeon, Young Ho | - |
dc.contributor.author | Hwang, Kwang Yeon | - |
dc.contributor.author | Kim, Sunghoon | - |
dc.contributor.author | Cho, Yunje | - |
dc.date.accessioned | 2021-09-05T03:57:58Z | - |
dc.date.available | 2021-09-05T03:57:58Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-10-21 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/97074 | - |
dc.description.abstract | In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC). Here, we report a crystal structure of the MSC subcomplex comprising ArgRS, glutaminyl-tRNA synthetase (GlnRS), and the auxiliary factor aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 (AIMP1)/p43. In this complex, the N-terminal domain of ArgRS forms a long coiled-coil structure with the N-terminal helix of AIMP1 and anchors the C-terminal core of GlnRS, thereby playing a central role in assembly of the three components. Mutation of AIMP1 destabilized the N-terminal helix of ArgRS and abrogated its catalytic activity. Mutation of the N-terminal helix of ArgRS liberated GlnRS, which is known to control cell death. This ternary complex was further anchored to AIMP2/p38 through interaction with AIMP1. These findings demonstrate the importance of interactions between the N-terminal domains of ArgRS and AIMP1 for the catalytic and noncatalytic activities of ArgRS and for the assembly of the higher-order MSC protein complex. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject | TRANSFER-RNA-SYNTHETASE | - |
dc.subject | AMINOACYL-TRANSFER-RNA | - |
dc.subject | PROTEIN-PROTEIN INTERACTIONS | - |
dc.subject | ORGANIZATION | - |
dc.subject | DISSECTION | - |
dc.subject | ARGININE | - |
dc.subject | REVEALS | - |
dc.subject | DOMAIN | - |
dc.title | Structure of the ArgRS-GlnRS-AIMP1 complex and its implications for mammalian translation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jeon, Young Ho | - |
dc.contributor.affiliatedAuthor | Hwang, Kwang Yeon | - |
dc.identifier.doi | 10.1073/pnas.1408836111 | - |
dc.identifier.scopusid | 2-s2.0-84908077920 | - |
dc.identifier.wosid | 000343302600043 | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.111, no.42, pp.15084 - 15089 | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.volume | 111 | - |
dc.citation.number | 42 | - |
dc.citation.startPage | 15084 | - |
dc.citation.endPage | 15089 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | TRANSFER-RNA-SYNTHETASE | - |
dc.subject.keywordPlus | AMINOACYL-TRANSFER-RNA | - |
dc.subject.keywordPlus | PROTEIN-PROTEIN INTERACTIONS | - |
dc.subject.keywordPlus | ORGANIZATION | - |
dc.subject.keywordPlus | DISSECTION | - |
dc.subject.keywordPlus | ARGININE | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordAuthor | arginyl-tRNA synthetase | - |
dc.subject.keywordAuthor | multisynthetase complex | - |
dc.subject.keywordAuthor | crystal structure | - |
dc.subject.keywordAuthor | AIMP1 | - |
dc.subject.keywordAuthor | glutaminyl-tRNA synthetase | - |
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