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Hyaluronic Acid-siRNA Conjugate/Reducible Polyethylenimine Complexes for Targeted siRNA Delivery
- Authors
- Jang, Yeon Lim; Ku, Sook Hee; Lee, So Jin; Park, Jae Hyung; Kim, Won Jong; Kwon, Ick Chan; Kim, Sun Hwa; Jeong, Ji Hoon
- Issue Date
- 10월-2014
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- siRNA Delivery; Hyaluronic Acid; Reducible Polyethylenimine; Tumor Targeting
- Citation
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.14, no.10, pp.7388 - 7394
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
- Volume
- 14
- Number
- 10
- Start Page
- 7388
- End Page
- 7394
- ISSN
- 1533-4880
- Abstract
- The clinical applications of therapeutic siRNA remain as a challenge due to the lack of efficient delivery system. In the present study, hyaluronic acid-siRNA conjugate (HA-SS-siRNA)/reducible polyethylenimine (BPEI1.2k-SS) complexes were developed to efficiently deliver the siRNA to HA receptor abundant region with the improved siRNA stability. HA and siRNA were conjugated with disulfide bonds, which are cleavable in cytoplasm. The synthesized HA-SS-siRNA was further complexed with BPEI1.2k-SS, resulting in the formation of spherical nanostructures with approximately 190 nm of size and neutral surface charge. HA-SS-siRNA/BPEI1.2k-SS complexes exhibited the improved stability against serum proteins or polyanions. These complexes were successfully translocated into intracellular region via HA receptor-mediated endocytosis, and silenced target gene expression.
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