Increased 2,3-butanediol production by changing codon usages in Escherichia coli
DC Field | Value | Language |
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dc.contributor.author | Park, Seo-Young | - |
dc.contributor.author | Kim, Borim | - |
dc.contributor.author | Lee, Soojin | - |
dc.contributor.author | Oh, Minkyu | - |
dc.contributor.author | Won, Jong-In | - |
dc.contributor.author | Lee, Jinwon | - |
dc.date.accessioned | 2021-09-05T05:41:04Z | - |
dc.date.available | 2021-09-05T05:41:04Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-09 | - |
dc.identifier.issn | 0885-4513 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/97520 | - |
dc.description.abstract | The natural microorganism Escherichia coli without modification is not suitable for the efficient production of 2,3-butanediol (2,3-BD) on an industrial scale because of its poor metabolic performance. Metabolic capacities of E. coli have been improved to produce 2,3-BD efficiently, the performance of which is possible for producing such a product. Codon optimization with the ribosome-binding site for the efficient production of target genes (budA and budC) was achieved by molecular engineering, which allowed the metabolic engineering to proceed to the next level. As a result, comparing the productivity in 26H, where the amount of p18COR was 1.04g/L and that of p18WTR was 0.41g/L, represents an approximate 60.6% increase in the productivity of the p18WTR with codon optimization. In other words, p18COR was 2.54-fold greater than p18WTR in the production of 2,3-BD. (C) 2014 International Union of Biochemistry and Molecular Biology, Inc. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | ALPHA-CYCLODEXTRIN GLYCOSYLTRANSFERASE | - |
dc.subject | PROTEIN-SYNTHESIS | - |
dc.subject | MESO-2,3-BUTANEDIOL | - |
dc.subject | OPTIMIZATION | - |
dc.subject | STATE | - |
dc.subject | RNA | - |
dc.title | Increased 2,3-butanediol production by changing codon usages in Escherichia coli | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Minkyu | - |
dc.identifier.doi | 10.1002/bab.1216 | - |
dc.identifier.scopusid | 2-s2.0-84923868558 | - |
dc.identifier.wosid | 000344174600006 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, v.61, no.5, pp.535 - 540 | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | - |
dc.citation.title | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | - |
dc.citation.volume | 61 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 535 | - |
dc.citation.endPage | 540 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | ALPHA-CYCLODEXTRIN GLYCOSYLTRANSFERASE | - |
dc.subject.keywordPlus | PROTEIN-SYNTHESIS | - |
dc.subject.keywordPlus | MESO-2,3-BUTANEDIOL | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | STATE | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | codon optimization | - |
dc.subject.keywordAuthor | ribosome-binding site | - |
dc.subject.keywordAuthor | 2 | - |
dc.subject.keywordAuthor | 3-butanediol | - |
dc.subject.keywordAuthor | acetolactate decarboxylase | - |
dc.subject.keywordAuthor | acetoin reductase | - |
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