Crystallization and preliminary X-ray analysis of the C-terminal fragment of Ski7 from Saccharomyces cerevisiae
DC Field | Value | Language |
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dc.contributor.author | Lee, Ji-Young | - |
dc.contributor.author | Park, Si Hoon | - |
dc.contributor.author | Jeong, Byung-Cheon | - |
dc.contributor.author | Song, Hyun Kyu | - |
dc.date.accessioned | 2021-09-05T05:46:49Z | - |
dc.date.available | 2021-09-05T05:46:49Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-09 | - |
dc.identifier.issn | 2053-230X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/97561 | - |
dc.description.abstract | Ski7 (superkiller protein 7) plays a critical role in the mRNA surveillance pathway. The C-terminal fragment of Ski7 (residues 520-747) from Saccharomyces cerevisiae was heterologously expressed in Escherichia coli and purified to homogeneity. It was successfully crystallized and preliminary X-ray data were collected to 2.0 angstrom resolution using synchrotron radiation. The crystal belonged to a trigonal space group, either P3(1)21 or P3(2)21, with unit-cell parameters a = b = 73.5, c = 83.6 angstrom. The asymmetric unit contains one molecule of the C-terminal fragment of Ski7 with a corresponding crystal volume per protein mass (V-M) of 2.61 angstrom(3) Da(-1) and a solvent content of 52.8% by volume. The merging R factor is 6.6%. Structure determination by MAD phasing is under way. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | INT UNION CRYSTALLOGRAPHY | - |
dc.subject | MESSENGER-RNA DECAY | - |
dc.subject | NO-GO | - |
dc.subject | TRANSLATION TERMINATION | - |
dc.subject | QUALITY-CONTROL | - |
dc.subject | FACTORS ERF1 | - |
dc.subject | COMPLEX | - |
dc.subject | YEAST | - |
dc.subject | DOMAIN | - |
dc.subject | ELONGATION | - |
dc.subject | EXOSOME | - |
dc.title | Crystallization and preliminary X-ray analysis of the C-terminal fragment of Ski7 from Saccharomyces cerevisiae | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Hyun Kyu | - |
dc.identifier.doi | 10.1107/S2053230X14016872 | - |
dc.identifier.scopusid | 2-s2.0-84907022378 | - |
dc.identifier.wosid | 000341818600026 | - |
dc.identifier.bibliographicCitation | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v.70, pp.1252 - 1255 | - |
dc.relation.isPartOf | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | - |
dc.citation.title | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | - |
dc.citation.volume | 70 | - |
dc.citation.startPage | 1252 | - |
dc.citation.endPage | 1255 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Crystallography | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Crystallography | - |
dc.subject.keywordPlus | MESSENGER-RNA DECAY | - |
dc.subject.keywordPlus | NO-GO | - |
dc.subject.keywordPlus | TRANSLATION TERMINATION | - |
dc.subject.keywordPlus | QUALITY-CONTROL | - |
dc.subject.keywordPlus | FACTORS ERF1 | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | ELONGATION | - |
dc.subject.keywordPlus | EXOSOME | - |
dc.subject.keywordAuthor | mRNA surveillance | - |
dc.subject.keywordAuthor | nonstop decay | - |
dc.subject.keywordAuthor | RNA degradation | - |
dc.subject.keywordAuthor | Saccharomyces cerevisiae | - |
dc.subject.keywordAuthor | Ski7 | - |
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