Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus
DC Field | Value | Language |
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dc.contributor.author | Kim, Tae-Yeon | - |
dc.contributor.author | Lee, Sang-Woo | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.date.accessioned | 2021-09-05T07:10:22Z | - |
dc.date.available | 2021-09-05T07:10:22Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-07 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/98003 | - |
dc.description.abstract | 2-Phenylethanol (2-PE) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-PE is produced in a few yeast strains by Ehrlich pathway. In this study, Kluyveromyces marxianus was genetically engineered for overproduction of 2-PE from glucose. About 1.0 g/L of 2-PE was produced by overexpressing phenylpyruvate decarboxylase (ARO10) and alcohol dehydrogenase (ADH2) genes of Saccharomyces cerevisiae. A similar level of 2-PE was also produced from evolved K. marxianus, which was resistant to the phenylalanine analog, p-fluorophenylalanine. aroG(fbr) from Klebsiella pneumoniae encoding a feedback resistant mutant of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DHAP) synthase was overexpressed in the evolved K. marxianus. Finally, 1.3 g/L of 2-PE was produced from 20 g/L glucose without addition of phenylalanine in the medium. (C) 2014 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | AROMA COMPOUND 2-PHENYLETHANOL | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | FEEDBACK INHIBITION | - |
dc.subject | DNA | - |
dc.subject | THERMOTOLERANT | - |
dc.subject | TRANSFORMATION | - |
dc.subject | ALCOHOLS | - |
dc.subject | ETHANOL | - |
dc.subject | YEASTS | - |
dc.subject | CHAIN | - |
dc.title | Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.1016/j.enzmictec.2014.04.011 | - |
dc.identifier.scopusid | 2-s2.0-84900804607 | - |
dc.identifier.wosid | 000338404400007 | - |
dc.identifier.bibliographicCitation | ENZYME AND MICROBIAL TECHNOLOGY, v.61-62, pp.44 - 47 | - |
dc.relation.isPartOf | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.title | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.volume | 61-62 | - |
dc.citation.startPage | 44 | - |
dc.citation.endPage | 47 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | AROMA COMPOUND 2-PHENYLETHANOL | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | FEEDBACK INHIBITION | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | THERMOTOLERANT | - |
dc.subject.keywordPlus | TRANSFORMATION | - |
dc.subject.keywordPlus | ALCOHOLS | - |
dc.subject.keywordPlus | ETHANOL | - |
dc.subject.keywordPlus | YEASTS | - |
dc.subject.keywordPlus | CHAIN | - |
dc.subject.keywordAuthor | 2-Phenylethanol | - |
dc.subject.keywordAuthor | DHAP synthase | - |
dc.subject.keywordAuthor | Kluyveromyces marxianus | - |
dc.subject.keywordAuthor | Ehrlich pathway | - |
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