The PDZ-binding motif of the avian NS1 protein affects transmission of the 2009 influenza A(H1N1) virus
- Authors
- Kim, Jin Il; Hwang, Min-Woong; Lee, Ilseob; Park, Sehee; Lee, Sangmoo; Bae, Joon-Yong; Heo, Jun; Kim, Donghwan; Jang, Seok-Il; Park, Mee Sook; Kwon, Hyung-Joo; Song, Jin-Won; Park, Man-Seong
- Issue Date
- 20-6월-2014
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Influenza A virus; Interferon; NS1 protein; PDZ-binding motif; Transmission
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.449, no.1, pp.19 - 25
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 449
- Number
- 1
- Start Page
- 19
- End Page
- 25
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/98210
- DOI
- 10.1016/j.bbrc.2014.04.132
- ISSN
- 0006-291X
- Abstract
- By nature of their segmented RNA genome, influenza A viruses (IAVs) have the potential to generate variants through a reassortment process. The influenza nonstructural (NS) gene is critical for a virus to counteract the antiviral responses of the host. Therefore, a newly acquired NS segment potentially determines the replication efficiency of the reassortant virus in a range of different hosts. In addition, the C-terminal PDZ-binding motif (PBM) has been suggested as a pathogenic determinant of IAVs. To gauge the pandemic potential from human and avian IAV reassortment, we assessed the replication properties of NS-reassorted viruses in cultured cells and in the lungs of mice and determined their transmissibility in guinea pigs. Compared with the recombinant A/Korea/01/2009 virus (rK09; 2009 pandemic H1N1 strain), the rK09/VN:NS virus, in which the NS gene was adopted from the A/Vietnam/1203/2004 virus (a human isolate of the highly pathogenic avian influenza H5N1 virus strains), exhibited attenuated virulence and reduced transmissibility. However, the rK09/VN:NS-PBM virus, harboring the PBM in the C-terminus of the NS1 protein, recovered the attenuated virulence of the rK09/VN:NS virus. In a guinea pig model, the rK09/VN:NS-PBM virus showed even greater transmission efficiency than the rK/09 virus. These results suggest that the PBM in the NS1 protein may determine viral persistence in the human and avian IAV interface. (C) 2014 Elsevier Inc. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.