HIF-1 alpha Expression as a Protective Strategy of HepG2 Cells Against Fatty Acid-Induced Toxicity
DC Field | Value | Language |
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dc.contributor.author | Yoo, Wonbaek | - |
dc.contributor.author | Noh, Kyung Hee | - |
dc.contributor.author | Ahn, Jae Hee | - |
dc.contributor.author | Yu, Ji Hee | - |
dc.contributor.author | Seo, Ji A. | - |
dc.contributor.author | Kim, Sin Gon | - |
dc.contributor.author | Choi, Kyung Mook | - |
dc.contributor.author | Baik, Sei Hyun | - |
dc.contributor.author | Choi, Dong Seop | - |
dc.contributor.author | Kim, Tae Woo | - |
dc.contributor.author | Kim, Hyo Joon | - |
dc.contributor.author | Kim, Nan Hee | - |
dc.date.accessioned | 2021-09-05T08:09:48Z | - |
dc.date.available | 2021-09-05T08:09:48Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-06 | - |
dc.identifier.issn | 0730-2312 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/98281 | - |
dc.description.abstract | Free fatty acid-induced lipotoxicity via increased endoplasmic reticulum (ER) stress and hepatocyte apoptosis is a key pathological mechanism of non-alcoholic steatohepatitis. A role of hypoxia-inducible factor 1 (HIF-1) in this process has been suggested, but direct evidence is lacking. Here, we used HepG2 cells as a model to study whether HIF-1 can reduce palmitic acid-induced lipotoxicity and ER stress. In HepG2 cells treated with 500 mu M palmitic acid, HIF-1 expression increased transiently, the decline was associated with increased cleaved caspase-3 expression. Overexpression and knockdown of HIF-1 decreased and exacerbated, respectively, palmitic acid-induced lipoapoptosis. The overexpression also blunted upregulation of the ER stress markers, C/EBP homologous protein (CHOP) and chaperone immunoglobulin heavy chain binding protein (Bip), while the knockdown increased the level of CHOP. In line with this, CHOP promoter activity decreased following HIF-1 binding to the CHOP promoter hypoxia response element. These results indicate that hepatocyte lipotoxicity is associated with decreased HIF-1 expression. It also suggests that upregulation of HIF-1 can be a possible strategy to reduce lipotoxicity in non-alcoholic fatty liver disease. J. Cell. Biochem. 115: 1147-1158, 2014. (c) 2014 Wiley Periodicals, Inc. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | ENDOPLASMIC-RETICULUM STRESS | - |
dc.subject | HYPOXIA-INDUCIBLE FACTOR-1-ALPHA | - |
dc.subject | OXIDE-INDUCED APOPTOSIS | - |
dc.subject | NITRIC-OXIDE | - |
dc.subject | HEPATOCYTE LIPOAPOPTOSIS | - |
dc.subject | LIPID-ACCUMULATION | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | LIVER-DISEASE | - |
dc.subject | GENE-THERAPY | - |
dc.subject | ER STRESS | - |
dc.title | HIF-1 alpha Expression as a Protective Strategy of HepG2 Cells Against Fatty Acid-Induced Toxicity | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yu, Ji Hee | - |
dc.contributor.affiliatedAuthor | Seo, Ji A. | - |
dc.contributor.affiliatedAuthor | Kim, Sin Gon | - |
dc.contributor.affiliatedAuthor | Choi, Kyung Mook | - |
dc.contributor.affiliatedAuthor | Baik, Sei Hyun | - |
dc.contributor.affiliatedAuthor | Choi, Dong Seop | - |
dc.contributor.affiliatedAuthor | Kim, Tae Woo | - |
dc.contributor.affiliatedAuthor | Kim, Nan Hee | - |
dc.identifier.doi | 10.1002/jcb.24757 | - |
dc.identifier.scopusid | 2-s2.0-84907422423 | - |
dc.identifier.wosid | 000334523300016 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELLULAR BIOCHEMISTRY, v.115, no.6, pp.1147 - 1158 | - |
dc.relation.isPartOf | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.citation.title | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.citation.volume | 115 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1147 | - |
dc.citation.endPage | 1158 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM STRESS | - |
dc.subject.keywordPlus | HYPOXIA-INDUCIBLE FACTOR-1-ALPHA | - |
dc.subject.keywordPlus | OXIDE-INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | HEPATOCYTE LIPOAPOPTOSIS | - |
dc.subject.keywordPlus | LIPID-ACCUMULATION | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | LIVER-DISEASE | - |
dc.subject.keywordPlus | GENE-THERAPY | - |
dc.subject.keywordPlus | ER STRESS | - |
dc.subject.keywordAuthor | NON-ALCOHOLIC FATTY LIVER DISEASE | - |
dc.subject.keywordAuthor | HEPATIC STEATOSIS | - |
dc.subject.keywordAuthor | LIPOAPOPTOSIS | - |
dc.subject.keywordAuthor | ER STRESS | - |
dc.subject.keywordAuthor | HIF-1 | - |
dc.subject.keywordAuthor | CHOP | - |
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