Alcohol induces cell proliferation via hypermethylation of ADHFE1 in colorectal cancer cells
DC Field | Value | Language |
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dc.contributor.author | Moon, Ji Wook | - |
dc.contributor.author | Lee, Soo Kyung | - |
dc.contributor.author | Lee, Yong Woo | - |
dc.contributor.author | Lee, Jung Ok | - |
dc.contributor.author | Kim, Nami | - |
dc.contributor.author | Lee, Hye Jeong | - |
dc.contributor.author | Seo, Jung Seon | - |
dc.contributor.author | Kim, Jin | - |
dc.contributor.author | Kim, Hyeon Soo | - |
dc.contributor.author | Park, Sun-Hwa | - |
dc.date.accessioned | 2021-09-05T08:42:47Z | - |
dc.date.available | 2021-09-05T08:42:47Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-05-28 | - |
dc.identifier.issn | 1471-2407 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/98487 | - |
dc.description.abstract | Background: The hypermethylation of Alcohol dehydrogenase iron containing 1 (ADHFE1) was recently reported to be associated with colorectal cancer (CRC) differentiation. However, the effect of alcohol on ADHFE1 hypermethylation in CRC is still unclear. Methods: The methylation status and expression levels of ADHFE1 were investigated in primary tumor tissues and adjacent normal tissues of 73 patients with CRC, one normal colon cell line, and 4 CRC cell lines (HT-29, SW480, DLD-1, and LoVo) by quantitative methylation-specific polymerase chain reaction (QMSP) and real-time reverse transcription polymerase chain reaction (real time PCR), respectively. The effect of alcohol on the methylation status of ADHFE1 was analyzed in HT-29, SW480, DLD-1, and CCD18Co cells using QMSP, real-time PCR, immunoblot, and cell proliferation assay. Results: ADHFE1 was hypermethylated in 69 of 73 CRC tissues (95%) compared to adjacent normal tissues (p < 0.05). The mRNA expression of ADHFE1 was significantly reduced in CRC compared to adjacent normal tissues (p < 0.05) and its expression was decreased in the alcohol consumption group (p < 0.05). ADHFE1 was hypermethylated and its expression was decreased in 4 CRC cell lines compared with normal colon cell line. Alcohol induced hypermethylation of ADHFE1, decreased its expression, and stimulated cell proliferation of HT-29, SW480, and DLD-1cells. Conclusion: These results demonstrate that the promoter hypermethylation of ADHFE1 is frequently present in CRC and alcohol induces methylation-mediated down expression of ADHFE1 and proliferation of CRC cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.subject | DNA METHYLATION CHANGES | - |
dc.subject | PROMOTER HYPERMETHYLATION | - |
dc.subject | EPIGENETIC INACTIVATION | - |
dc.subject | GROWTH-FACTOR | - |
dc.subject | COLON | - |
dc.subject | GENE | - |
dc.subject | DEHYDROGENASE | - |
dc.subject | LUNG | - |
dc.title | Alcohol induces cell proliferation via hypermethylation of ADHFE1 in colorectal cancer cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jin | - |
dc.contributor.affiliatedAuthor | Kim, Hyeon Soo | - |
dc.contributor.affiliatedAuthor | Park, Sun-Hwa | - |
dc.identifier.doi | 10.1186/1471-2407-14-377 | - |
dc.identifier.scopusid | 2-s2.0-84902364121 | - |
dc.identifier.wosid | 000338150700001 | - |
dc.identifier.bibliographicCitation | BMC CANCER, v.14 | - |
dc.relation.isPartOf | BMC CANCER | - |
dc.citation.title | BMC CANCER | - |
dc.citation.volume | 14 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | DNA METHYLATION CHANGES | - |
dc.subject.keywordPlus | PROMOTER HYPERMETHYLATION | - |
dc.subject.keywordPlus | EPIGENETIC INACTIVATION | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | COLON | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | DEHYDROGENASE | - |
dc.subject.keywordPlus | LUNG | - |
dc.subject.keywordAuthor | ADHFE1 | - |
dc.subject.keywordAuthor | Colorectal cancer | - |
dc.subject.keywordAuthor | Hypermethylation | - |
dc.subject.keywordAuthor | Alcohol | - |
dc.subject.keywordAuthor | Quantitative methylation-specific polymerase chain reaction | - |
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