DNA Amplification in Neutral Liposomes for Safe and Efficient Gene Delivery
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Sangmin | - |
dc.contributor.author | Koo, Heebeom | - |
dc.contributor.author | Na, Jin Hee | - |
dc.contributor.author | Lee, Kyung Eun | - |
dc.contributor.author | Jeong, Seo Young | - |
dc.contributor.author | Choi, Kuiwon | - |
dc.contributor.author | Kim, Sun Hwa | - |
dc.contributor.author | Kwon, Ick Chan | - |
dc.contributor.author | Kim, Kwangmeyung | - |
dc.date.accessioned | 2021-09-05T09:07:00Z | - |
dc.date.available | 2021-09-05T09:07:00Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-05 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/98612 | - |
dc.description.abstract | In general, traditional gene carriers contain strong cationic charges to efficiently load anionic genes, but this cationic character also leads to destabilization of plasma membranes and causes severe cytotoxicity. Here, we developed a PCR-based nanofactory as a safe gene delivery system. A few template plasmid DNA can be amplified by PCR inside liposomes about 200 nm in diameter, and the quantity of loaded genes highly Increased by more than 8.8-fold. The liposome membrane was composed of neutral lipids free from cationic charges. Consequently, this system is nontoxic, unlike other traditional cationic gene carriers. Intense red fluorescent protein (REP) expression in CHO-K1 cells showed that the amplified genes could be successfully transfected to cells. Animal experiments with the luciferase gene also showed in vivo gene expression by our system without toxicity. We think that this PCR-based nanofactory system can overcome the toxicity problem that is the critical limitation of current gene delivery to clinical application. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | TARGETED DELIVERY | - |
dc.subject | IN-VIVO | - |
dc.subject | PROTEIN | - |
dc.subject | NANOPARTICLES | - |
dc.subject | THERAPY | - |
dc.title | DNA Amplification in Neutral Liposomes for Safe and Efficient Gene Delivery | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kwon, Ick Chan | - |
dc.identifier.doi | 10.1021/nn501106a | - |
dc.identifier.scopusid | 2-s2.0-84901659599 | - |
dc.identifier.wosid | 000336640600017 | - |
dc.identifier.bibliographicCitation | ACS NANO, v.8, no.5, pp.4257 - 4267 | - |
dc.relation.isPartOf | ACS NANO | - |
dc.citation.title | ACS NANO | - |
dc.citation.volume | 8 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 4257 | - |
dc.citation.endPage | 4267 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.subject.keywordPlus | TARGETED DELIVERY | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordAuthor | polymerase chain reaction | - |
dc.subject.keywordAuthor | neutral liposomes | - |
dc.subject.keywordAuthor | gene delivery | - |
dc.subject.keywordAuthor | nanofactory | - |
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