Evaluation of Propidium Monoazide Real-Time PCR for Early Detection of Viable Mycobacterium tuberculosis in Clinical Respiratory Specimens
DC Field | Value | Language |
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dc.contributor.author | Kim, Young Jin | - |
dc.contributor.author | Lee, Sun Min | - |
dc.contributor.author | Park, Byung Kyu | - |
dc.contributor.author | Kim, Sung Soo | - |
dc.contributor.author | Yi, Jongyoun | - |
dc.contributor.author | Kim, Hyung Hoi | - |
dc.contributor.author | Lee, Eun Yup | - |
dc.contributor.author | Chang, Chulhun Ludgerus | - |
dc.date.accessioned | 2021-09-05T09:08:29Z | - |
dc.date.available | 2021-09-05T09:08:29Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-05 | - |
dc.identifier.issn | 2234-3806 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/98623 | - |
dc.description.abstract | Background: Conventional acid-fast bacilli (AFB) staining cannot differentiate viable from dead cells. Propidium monoazide (PMA) is a photoreactive DNA-binding dye that inhibits PCR amplification by DNA modification. We evaluated whether PMA real-time PCR is suitable for the early detection of viable Mycobacterium tuberculosis (MTB) in clinical respiratory specimens. Methods: A total of 15 diluted suspensions from 5 clinical MTB isolates were quadruplicated and subjected to PMA treatment and/or heat inactivation. Eighty-three AFB-positive sputum samples were also tested to compare the ACT values (CT value in PMA-treated sputum samples CT value in non-PMA-treated sputum samples) between culture-positive and culture-negative specimens. Real-time PCR was performed using Anyplex MTB/NTM Real-Time Detection (Seegene, Korea), and the CT value changes after PMA treatment were compared between culture-positive and culture-negative groups. Results: In MTB suspensions, the increase in the Cr value after PMA treatment was significant in dead cells (P=0.0001) but not in live cells (P=0.1070). In 14 culture-negative sputum samples, the median ACT value was 5.3 (95% confidence interval [Cl], 4.1-8.2; P<0.0001), whereas that in 69 culture-positive sputum samples was 1.1 (95% Cl, 0.72.0). In the ROC curve analysis, the cutoff ACT value for maximum sensitivity (89.9%) and specificity (85.7%) for differentiating dead from live cells was 3.4. Conclusions: PMA real-time PCR is a useful approach for differentiating dead from live bacilli in AFB smear-positive sputum samples. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC LABORATORY MEDICINE | - |
dc.subject | SELECTIVE DETECTION | - |
dc.subject | DIAGNOSIS | - |
dc.subject | CELLS | - |
dc.title | Evaluation of Propidium Monoazide Real-Time PCR for Early Detection of Viable Mycobacterium tuberculosis in Clinical Respiratory Specimens | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Byung Kyu | - |
dc.identifier.doi | 10.3343/alm.2014.34.3.203 | - |
dc.identifier.scopusid | 2-s2.0-84900460171 | - |
dc.identifier.wosid | 000335621700004 | - |
dc.identifier.bibliographicCitation | ANNALS OF LABORATORY MEDICINE, v.34, no.3, pp.203 - 209 | - |
dc.relation.isPartOf | ANNALS OF LABORATORY MEDICINE | - |
dc.citation.title | ANNALS OF LABORATORY MEDICINE | - |
dc.citation.volume | 34 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 203 | - |
dc.citation.endPage | 209 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001872374 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Medical Laboratory Technology | - |
dc.relation.journalWebOfScienceCategory | Medical Laboratory Technology | - |
dc.subject.keywordPlus | SELECTIVE DETECTION | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | Mycobacterium tuberculosis | - |
dc.subject.keywordAuthor | Propidium monoazide | - |
dc.subject.keywordAuthor | Real-time PCR | - |
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