Analysis of cepA Encoding an Efflux Pump-like Protein in Corynebacterium glutamicum
DC Field | Value | Language |
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dc.contributor.author | Sim, Soo-Yeon | - |
dc.contributor.author | Hong, Eun-Ji | - |
dc.contributor.author | Kim, Younhee | - |
dc.contributor.author | Lee, Heung-Shick | - |
dc.date.accessioned | 2021-09-05T10:08:04Z | - |
dc.date.available | 2021-09-05T10:08:04Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-04 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/98875 | - |
dc.description.abstract | A gene encoding a homolog of purine efflux proteins of Escherichia coli and Bacillus subtilis was identified in the genome of Corynebacterium glutamicum and designated as cepA. The gene encoded a putative protein product, containing 12 transmembrane helixes, which is a typical feature of integral membrane transport proteins. To elucidate the function of the gene, we constructed a cepA deletion mutant (Delta cepA) and a cepA-overexpressing strain and analyzed their physiological characteristics. The cepA gene could be deleted with no critical effect on cell growth. However, the cell yield of a Delta cepA strain was decreased by 10% as compared to that of a strain carrying a cepA-overexpression plasmid (P-180-cepA). Further analysis identified increased resistance of the P-180-cepA strain to the purine analogues 6-mercaptopurine and 6-mercaptoguanine, but not to 2-aminopurine and purine nucleoside analogues. Moreover, this strain showed increased resistance to the antibiotics nalidixic acid and ampicillin. Collectively, these data suggest that cepA is a novel multidrug resistance gene and probably functions in the efflux of toxic substances from the inside of cells to the environment, thus allowing cells to reach a higher cell yield. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | GENOME SEQUENCE | - |
dc.subject | AMINO-ACIDS | - |
dc.subject | GENE | - |
dc.subject | RESISTANCE | - |
dc.subject | PBUE | - |
dc.subject | EXPRESSION | - |
dc.subject | BACTERIA | - |
dc.subject | CLONING | - |
dc.subject | EXPORT | - |
dc.title | Analysis of cepA Encoding an Efflux Pump-like Protein in Corynebacterium glutamicum | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Heung-Shick | - |
dc.identifier.doi | 10.1007/s12275-014-3461-1 | - |
dc.identifier.scopusid | 2-s2.0-84897424025 | - |
dc.identifier.wosid | 000333574600003 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.52, no.4, pp.278 - 283 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 52 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 278 | - |
dc.citation.endPage | 283 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001864761 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GENOME SEQUENCE | - |
dc.subject.keywordPlus | AMINO-ACIDS | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | PBUE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | EXPORT | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordAuthor | cepA | - |
dc.subject.keywordAuthor | efflux | - |
dc.subject.keywordAuthor | purine | - |
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