Involvement of caspase-2 activation in aurora kinase inhibitor-induced cell death in axin-expressing L929 cells
DC Field | Value | Language |
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dc.contributor.author | Choi, Eun-Jin | - |
dc.contributor.author | Kim, Shi-Mun | - |
dc.contributor.author | Shin, Jee-Hye | - |
dc.contributor.author | Kim, Sewon | - |
dc.contributor.author | Song, Ki-Joon | - |
dc.contributor.author | Kee, Sun-Ho | - |
dc.date.accessioned | 2021-09-05T10:13:34Z | - |
dc.date.available | 2021-09-05T10:13:34Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-04 | - |
dc.identifier.issn | 1360-8185 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/98914 | - |
dc.description.abstract | Axin is a multifunctional protein that participates in many cellular events including Wnt signaling and cell fate determination. Aurora kinase inhibitor (AKI)-induced cell death and cell membrane rupture is facilitated in L929 cells expressing axin (L-axin cells) through the activation of poly ADP-ribose polymerase (PARP). We observed that caspase-2 activity is required for AKI-induced cell death. Inhibition of caspase-2 activity suppressed AKI-induced PARP activation and mitochondrial dysfunction, resulting in a decrease in AKI-induced cell death. When an axin mutant deleted for the glycogen synthase kinase 3 beta (GSK3 beta)-binding domain was expressed in L929 cells (L-Delta GSK cells), AKI-induced caspase-2 activation and cell death decreased. AKI treatment reduced the expression of a 32-kDa caspase-2 splicing variant (caspase-2S) in most L-axin cells, but not in L-Delta GSK cells. These results suggest that AKI-induced caspase-2 activation in L-axin cells might be due to a decrease in the expression of caspase-2S, which inhibits caspase-2 activity. In addition, AKI treatment failed to activate caspase-8 and treatment with necrostatin inhibited AKI-induced cell death in L-axin cells, suggesting that the absence of caspase-8 activation might favor necrotic cell death. Axin expression may facilitate AKI-induced caspase-2 activation followed by activation of PARP and initiation of the necrotic cell death pathway. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | INDUCED APOPTOSIS | - |
dc.subject | PHYSICAL INTERACTION | - |
dc.subject | MEDIATED APOPTOSIS | - |
dc.subject | BODY FORMATION | - |
dc.subject | CANCER-CELLS | - |
dc.subject | DNA-DAMAGE | - |
dc.subject | IN-VITRO | - |
dc.subject | P53 | - |
dc.subject | MITOCHONDRIA | - |
dc.subject | PROTEASE | - |
dc.title | Involvement of caspase-2 activation in aurora kinase inhibitor-induced cell death in axin-expressing L929 cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Jee-Hye | - |
dc.contributor.affiliatedAuthor | Song, Ki-Joon | - |
dc.contributor.affiliatedAuthor | Kee, Sun-Ho | - |
dc.identifier.doi | 10.1007/s10495-013-0951-2 | - |
dc.identifier.scopusid | 2-s2.0-84896401553 | - |
dc.identifier.wosid | 000332485700009 | - |
dc.identifier.bibliographicCitation | APOPTOSIS, v.19, no.4, pp.657 - 667 | - |
dc.relation.isPartOf | APOPTOSIS | - |
dc.citation.title | APOPTOSIS | - |
dc.citation.volume | 19 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 657 | - |
dc.citation.endPage | 667 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | PHYSICAL INTERACTION | - |
dc.subject.keywordPlus | MEDIATED APOPTOSIS | - |
dc.subject.keywordPlus | BODY FORMATION | - |
dc.subject.keywordPlus | CANCER-CELLS | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | P53 | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordPlus | PROTEASE | - |
dc.subject.keywordAuthor | Axin | - |
dc.subject.keywordAuthor | Wnt signaling | - |
dc.subject.keywordAuthor | Aurora kinase | - |
dc.subject.keywordAuthor | Caspase-2 | - |
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