Valproic acid promotes differentiation of hepatocyte-like cells from whole human umbilical cord-derived mesenchymal stem cells
- Authors
- An, Su Yeon; Han, Jiyou; Lim, Hee-Joung; Park, Seo-Young; Kim, Ji Hyang; Do, Byung-Rok; Kim, Jong-Hoon
- Issue Date
- 4월-2014
- Publisher
- CHURCHILL LIVINGSTONE
- Keywords
- Mesenchymal stem cells; Endoderm; Differentiation; AKT; ERK
- Citation
- TISSUE & CELL, v.46, no.2, pp.127 - 135
- Indexed
- SCIE
SCOPUS
- Journal Title
- TISSUE & CELL
- Volume
- 46
- Number
- 2
- Start Page
- 127
- End Page
- 135
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/98926
- DOI
- 10.1016/j.tice.2013.12.006
- ISSN
- 0040-8166
- Abstract
- Mesenchymal stem cells (MSCs) are mesoderm-derived cells that are considered a good source of somatic cells for treatment of many degenerative diseases. Previous studies have reported the differentiation of mesodermal MSCs into endodermal and ectodermal cell types beyond their embryonic lineages, including hepatocytes and neurons. However, the molecular pathways responsible for the direct or indirect cell type conversion and the functional ability of the differentiated cells remain unclear and need further research. In the present study, we demonstrated that valproic acid (VPA), which is a histone deacetylase inhibitor, induced an increase in the expression of endodermal genes including CXCR4, SOX17, FOXA1, FOXA2, GSC, c-MET, EOMES, and HNF-1 beta in human umbilical cord derived MSCs (hUCMSCs). In addition, we found that VPA is able to increase these endodermal genes in hUCMSCs by activating signal transduction of AKT and ERK. VPA pretreatment increased hepatic differentiation at the expense of adipogenic differentiation. The effects of VPA on modulating hUCMSCs fate were diminished by blocking AKT and ERK activation using specific signaling inhibitors. Together, our results suggest that VPA contributes to the lineage conversion of hUCMSCs to hepatic cell fate by upregulating the expression of endodermal genes through AKT and ERK activation. (C) 2013 Elsevier Ltd. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.