Local delivery of recombinant human bone morphogenic protein-2 (rhBMP-2) from rhBMP-2/heparin complex fixed to a chitosan scaffold enhances osteoblast behavior
DC Field | Value | Language |
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dc.contributor.author | Yun, Young-Pil | - |
dc.contributor.author | Yang, Dae Hyeok | - |
dc.contributor.author | Kim, Sun-Wook | - |
dc.contributor.author | Park, Kyeongsoon | - |
dc.contributor.author | Ohe, Joo-Young | - |
dc.contributor.author | Lee, Baek-Soo | - |
dc.contributor.author | Choi, Byung-Joon | - |
dc.contributor.author | Kim, Sung Eun | - |
dc.date.accessioned | 2021-09-05T10:19:08Z | - |
dc.date.available | 2021-09-05T10:19:08Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-04 | - |
dc.identifier.issn | 1738-2696 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/98950 | - |
dc.description.abstract | The objective of this study was to determine the effects on osteoblast activity of a recombinant human bone morphogenic protein (rhBMP)-2/heparin complex immobilized on a chitosan scaffold (Hep-C-rhBMP-2/chitosan) compared to a rhBMP-2-dipping chitosan (rhBMP-2/chitosan) and to a chitosan scaffold alone. Scanning electron microscopy (SEM) confirmed that the surface and cross morphologies of Hep-C-rhBMP-2/chitosan and rhBMP-2/chitosan were similar to those of the chitosan scaffold, and that the Hep-C-rhBMP-2/chitosan scaffold achieved sustained, controlled release of rhBMP-2 compared to the rhBMP-2/chitosan scaffold. We used measurements of alkaline phosphatase activity, calcium deposition, and real-time PCR to determine that significantly more MG-63 cells were successfully cultured on Hep-C-rhBMP-2/chitosan than on rhBMP-2/chitosan or the chitosan scaffold alone. These results suggest that the Hep-C-rhBMP-2/chitosan scaffold promotes osteoblast behavior beyond the other two scaffolds tested. This system may have advantages for dental and orthopedic applications to regenerate bone. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC | - |
dc.subject | MESENCHYMAL STEM-CELLS | - |
dc.subject | MARROW STROMAL CELLS | - |
dc.subject | ACID-BASED HYDROGEL | - |
dc.subject | SURFACE FUNCTIONALIZATION | - |
dc.subject | COMPOSITE SCAFFOLD | - |
dc.subject | MOLECULAR-WEIGHT | - |
dc.subject | GROWTH-FACTOR | - |
dc.subject | IN-VITRO | - |
dc.subject | HEPARIN | - |
dc.subject | TITANIUM | - |
dc.title | Local delivery of recombinant human bone morphogenic protein-2 (rhBMP-2) from rhBMP-2/heparin complex fixed to a chitosan scaffold enhances osteoblast behavior | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Sung Eun | - |
dc.identifier.doi | 10.1007/s13770-014-0049-0 | - |
dc.identifier.scopusid | 2-s2.0-84898439191 | - |
dc.identifier.wosid | 000334179000010 | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.11, no.2, pp.163 - 170 | - |
dc.relation.isPartOf | TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.citation.title | TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.citation.volume | 11 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 163 | - |
dc.citation.endPage | 170 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001862971 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.subject.keywordPlus | MESENCHYMAL STEM-CELLS | - |
dc.subject.keywordPlus | MARROW STROMAL CELLS | - |
dc.subject.keywordPlus | ACID-BASED HYDROGEL | - |
dc.subject.keywordPlus | SURFACE FUNCTIONALIZATION | - |
dc.subject.keywordPlus | COMPOSITE SCAFFOLD | - |
dc.subject.keywordPlus | MOLECULAR-WEIGHT | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | HEPARIN | - |
dc.subject.keywordPlus | TITANIUM | - |
dc.subject.keywordAuthor | chitosan scaffold | - |
dc.subject.keywordAuthor | recombinant human bone morphogenic protein-2 ( rhBMP-2) | - |
dc.subject.keywordAuthor | heparin | - |
dc.subject.keywordAuthor | enhancement of | - |
dc.subject.keywordAuthor | osteoblast behavior | - |
dc.subject.keywordAuthor | bone tissue engineering | - |
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