Serotonin-2C receptor involved serotonin-induced Ca2+ mobilisations in neuronal progenitors and neurons in rat suprachiasmatic nucleus
DC Field | Value | Language |
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dc.contributor.author | Takeuchi, Kouhei | - |
dc.contributor.author | Mohammad, Shahid | - |
dc.contributor.author | Ozaki, Tomoya | - |
dc.contributor.author | Morioka, Eri | - |
dc.contributor.author | Kawaguchi, Kaori | - |
dc.contributor.author | Kim, Juhyon | - |
dc.contributor.author | Jeong, Byeongha | - |
dc.contributor.author | Hong, Jin Hee | - |
dc.contributor.author | Lee, Kyoung J. | - |
dc.contributor.author | Ikeda, Masayuki | - |
dc.date.accessioned | 2021-09-05T11:20:25Z | - |
dc.date.available | 2021-09-05T11:20:25Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-02-17 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/99274 | - |
dc.description.abstract | The hypothalamic suprachiasmatic nucleus (SCN), the central circadian pacemaker in mammals, undergoes serotonergic regulation, but the underlying mechanisms remain obscure. Here, we generated a subclone of an SCN progenitor cell line expressing Ca(2+)sensors (SCN2.2YC) and compared its 5-HT receptor signalling with that of rat SCN neurons in brain slices. SCN2.2YC cells expressed 5-HT1A/2A/2B/2C, but not 5A/7, while all six subtypes were expressed in SCN tissues. HighK(+) or 5-HT increased cytosolic Ca2+ in SCN2.2YC cells. The 5-HT responses were inhibited by ritanserin and SB-221284, but resistant to WAY-100635 and RS-127445, suggesting predominant involvement of 5-HT2C for Ca2+ mobilisations. Consistently, Ca2+ imaging and voltage-clamp electrophysiology using rat SCN slices demonstrated post-synaptic 5-HT2C expression. Because 5-HT2C expression was postnatally increased in the SCN and 5-HT-induced Ca2+ mobilisations were amplified in differentiated SCN2.2YC cells and developed SCN neurons, we suggest that this signalling development occurs in accordance with central clock maturations. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | CIRCADIAN GENE-EXPRESSION | - |
dc.subject | INDUCED PHASE-SHIFTS | - |
dc.subject | C-FOS | - |
dc.subject | CELL-LINES | - |
dc.subject | ACTIVITY RHYTHM | - |
dc.subject | KNOCKOUT MICE | - |
dc.subject | IN-VITRO | - |
dc.subject | SCN | - |
dc.subject | HAMSTER | - |
dc.subject | AGONIST | - |
dc.title | Serotonin-2C receptor involved serotonin-induced Ca2+ mobilisations in neuronal progenitors and neurons in rat suprachiasmatic nucleus | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Kyoung J. | - |
dc.identifier.doi | 10.1038/srep04106 | - |
dc.identifier.scopusid | 2-s2.0-84894057359 | - |
dc.identifier.wosid | 000331398800002 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.4 | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 4 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | CIRCADIAN GENE-EXPRESSION | - |
dc.subject.keywordPlus | INDUCED PHASE-SHIFTS | - |
dc.subject.keywordPlus | C-FOS | - |
dc.subject.keywordPlus | CELL-LINES | - |
dc.subject.keywordPlus | ACTIVITY RHYTHM | - |
dc.subject.keywordPlus | KNOCKOUT MICE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | SCN | - |
dc.subject.keywordPlus | HAMSTER | - |
dc.subject.keywordPlus | AGONIST | - |
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