Neuroprotective biflavonoids of Chamaecyparis obtusa leaves against glutamate-induced oxidative stress in HT22 hippocampal cells
- Authors
- Jeong, Eun Ju; Hwang, Lim; Lee, Mina; Lee, Ki Yong; Ahn, Mi-Jeong; Sung, Sang Hyun
- Issue Date
- 2월-2014
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Chamaecyparis obtusa; Biflavonoid; Neuroprotection; Oxidative stress; HT22; ERK
- Citation
- FOOD AND CHEMICAL TOXICOLOGY, v.64, pp.397 - 402
- Indexed
- SCIE
SCOPUS
- Journal Title
- FOOD AND CHEMICAL TOXICOLOGY
- Volume
- 64
- Start Page
- 397
- End Page
- 402
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/99377
- DOI
- 10.1016/j.fct.2013.12.003
- ISSN
- 0278-6915
- Abstract
- Four biflavonoids (1-4), five flavonoids glycosides (5-9), two catechins (10, 11), two lignans (12-13), neolignan glycoside (14) and phenylpropanoid glycoside (15) were isolated from the leaves of Chamaecyparis obtusa (Cupressaceae). Neuroprotective effects of the isolated compounds were evaluated employing HT22 mouse hippocampal cells, a model system to study glutamate-induced oxidative stress. The glutamate injured HT22 cells were protected significantly by amentoflavone (3), ginkgetin (4) and (-)-epitaxifolin 3-O-beta-D-xylopyranoside (9). The reduced activities of antioxidant enzymes, superoxide dismutase (SOD), glutathione reductase (GR) in response to high concentration of glutamate were preserved by pre-treatment of 3,4 or 9, while the activities of glutathione peroxidase (Gpx) and catalase (CAT) were little affected. The reduced content of GSH induced by glutamate was also recovered by 3, 4 or 9 in accommodation with the decrease in ROS production. In addition, the phosphorylation of ERK1/2 induced by glutamate insult was clearly prevented by 3, while little changed by 4. Taken together, amentoflavone (3), ginkgetin (4) and (-)-epitaxifolin 3-O-beta-D-xylopyranoside (9) derived from C obtusa could protect HT22 neuronal cells against glutamate-induced oxidative damage through preserving antioxidant enzymes activities and/or inhibiting ERK1/2 activation. (C) 2013 Elsevier Ltd. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Pharmacy > Department of Pharmaceutical Science > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.