Effects of secreted factors in culture medium of annulus fibrosus cells on microvascular endothelial cells: elucidating the possible pathomechanisms of matrix degradation and nerve in-growth in disc degeneration
DC Field | Value | Language |
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dc.contributor.author | Moon, H. J. | - |
dc.contributor.author | Yurube, T. | - |
dc.contributor.author | Lozito, T. P. | - |
dc.contributor.author | Pohl, P. | - |
dc.contributor.author | Hartman, R. A. | - |
dc.contributor.author | Sowa, G. A. | - |
dc.contributor.author | Kang, J. D. | - |
dc.contributor.author | Vo, N. V. | - |
dc.date.accessioned | 2021-09-05T11:43:40Z | - |
dc.date.available | 2021-09-05T11:43:40Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-02 | - |
dc.identifier.issn | 1063-4584 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/99413 | - |
dc.description.abstract | Objective: To test whether the interaction between annulus fibrosus cells (AFCs) and endothelial cells (ECs) disrupts matrix homeostasis and stimulates production of innervation mediators. Methods: Human microvascular ECs were cultured in the conditioned media of AF cell culture derived from degenerated human surgical specimen. Matrix-metalloproteinases (MMPs) and platelet-derived growth factor (PDGF) of ECs of this culture were analyzed- by qRT-PCR, Western, and immunofluorescence. Vascular endothelial growth factor (VEGF), Interleukin-8 (IL-8), and nerve growth factor (NGF) in the media of this cell culture were assayed by ELISA. To determine the effects of ECs on AFCs, qRT-PCR was performed to determine mRNA levels of collagen I, II and aggrecan in AFCs cultured in EC conditioned media. Results: Compared to ECs cultured in na ve media, ECs exposed to AFC conditioned media expressed higher mRNA and protein levels of key biomarkers of invasive EC phenotype, MMP-2 (2x), MMP-13 (4x), and PDGF-B (1.5-2 x), and NGF (24.9 15.2 pg/mL vs 0 in na ve media). Treatment of AF cells with EC culture conditioned media decreased collagen type II expression two fold. Considerable quantities of proangiogenic factors IL-8 (396.7 302.0 pg/mL) and VEGF (756.2 375.9 pg/mL) were also detected in the conditioned media of untreated AF cell culture. Discussion: AFCs from degenerated discs secreted factors which stimulated EC production of factors known to induce matrix degradation, angiogenesis, and innervation. IL-8 and VEGF maybe the secreted factors from AFCs which mediate a pro-angiogenic stimulus often implicated in the development of disc degeneration. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | HUMAN INTERVERTEBRAL DISC | - |
dc.subject | OSTEOCHONDRAL JUNCTION | - |
dc.subject | PDGF-BB | - |
dc.subject | METALLOPROTEINASES | - |
dc.subject | ANGIOGENESIS | - |
dc.subject | COLLAGEN | - |
dc.subject | EXPRESSION | - |
dc.subject | AGE | - |
dc.subject | ACTIVATION | - |
dc.subject | LOCALIZATION | - |
dc.title | Effects of secreted factors in culture medium of annulus fibrosus cells on microvascular endothelial cells: elucidating the possible pathomechanisms of matrix degradation and nerve in-growth in disc degeneration | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Moon, H. J. | - |
dc.identifier.doi | 10.1016/j.joca.2013.12.008 | - |
dc.identifier.scopusid | 2-s2.0-84893781652 | - |
dc.identifier.wosid | 000332347600022 | - |
dc.identifier.bibliographicCitation | OSTEOARTHRITIS AND CARTILAGE, v.22, no.2, pp.344 - 354 | - |
dc.relation.isPartOf | OSTEOARTHRITIS AND CARTILAGE | - |
dc.citation.title | OSTEOARTHRITIS AND CARTILAGE | - |
dc.citation.volume | 22 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 344 | - |
dc.citation.endPage | 354 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Orthopedics | - |
dc.relation.journalResearchArea | Rheumatology | - |
dc.relation.journalWebOfScienceCategory | Orthopedics | - |
dc.relation.journalWebOfScienceCategory | Rheumatology | - |
dc.subject.keywordPlus | HUMAN INTERVERTEBRAL DISC | - |
dc.subject.keywordPlus | OSTEOCHONDRAL JUNCTION | - |
dc.subject.keywordPlus | PDGF-BB | - |
dc.subject.keywordPlus | METALLOPROTEINASES | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | COLLAGEN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | AGE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.subject.keywordAuthor | Intervertebral disc degeneration | - |
dc.subject.keywordAuthor | Angiogenesis | - |
dc.subject.keywordAuthor | Tip cells | - |
dc.subject.keywordAuthor | Annulus fibrosus | - |
dc.subject.keywordAuthor | Endothelial cells | - |
dc.subject.keywordAuthor | Metalloproteinases | - |
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