CXXC5 is a transcriptional activator of Flk-1 and mediates bone morphogenic protein-induced endothelial cell differentiation and vessel formation
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyun-Yi | - |
dc.contributor.author | Yang, Dong-Hwa | - |
dc.contributor.author | Shin, Song-Weon | - |
dc.contributor.author | Kim, Mi-Yeon | - |
dc.contributor.author | Yoon, Jae-Hyun | - |
dc.contributor.author | Kim, Suhyun | - |
dc.contributor.author | Park, Hae-Chul | - |
dc.contributor.author | Kang, Dong Woo | - |
dc.contributor.author | Min, DoSik | - |
dc.contributor.author | Hur, Man-Wook | - |
dc.contributor.author | Choi, Kang-Yell | - |
dc.date.accessioned | 2021-09-05T11:55:29Z | - |
dc.date.available | 2021-09-05T11:55:29Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-02 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/99457 | - |
dc.description.abstract | CXXC5 is a member of a small subset of proteins containing CXXC-type zinc-finger domain. Here, we show that CXXC5 is a transcription factor activating Flk-1, a receptor for vascular endothelial growth factor. CXXC5 and Flk-1 were accmulated in nucli and membrane of mouse embryonic stem cells (mESCs), respectively, during their endothelial differentiation. CXXC5 overexpression induced Flk-1 transcription in both endothelium-differentiated mESCs and human umbilical vein endothelial cells (HUVECs). In vitro DNA binding assay showed direct interaction of CXXC5 on the Flk-1 promoter region, and mutation on its DNA-binding motif abolished transcriptional activity. We showed that bone morphorgeneic protein 4 (BMP4) induced CXXC5 transcription in the cells, and inhibitors of BMP signaling suppressed the CXXC5 induction and the consequent Flk-1 induction by BMP4 treatment. CXXC5 knockdown resulted in suppression of BMP4-induced stress fiber formation (56.8 +/- 1.3% decrease, P<0.05) and migration (54.6 +/- 1.9% decrease, P<0.05) in HUVECs. The in vivo roles of CXXC5 in BMP-signaling-specific vascular development and angiogenesis were shown by specific defect of caudal vein plex vessel formation (57.9 +/- 11.8% decrease, P<0.05) in cxxc5 morpholino-injected zebrafish embryos and by supression of BMP4-induced angigogensis in subcutaneously injected Matrigel plugs in CXXC5(-/-) mice. Overall, CXXC5 is a transcriptional activator for Flk-1, mediating BMP signaling for differentiation and migration of endothelial cell and vessel formation.Kim, H.-Y., Yang, D.-H., Shin, S.-W., Kim, M.-Y., Yoon, J.-H., Kim, S., Park, H.-C., Kang, D. W., Min, D., Hur, M.-W., Choi, K.-Y. CXXC5 is a transcriptional activator of Flk-1 and mediates bone morphogenic protein-induced endothelial cell differentiation and vessel formation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | FEDERATION AMER SOC EXP BIOL | - |
dc.subject | EMBRYONIC STEM-CELLS | - |
dc.subject | SIGNAL-TRANSDUCTION | - |
dc.subject | BINDING DOMAIN | - |
dc.subject | VEGF | - |
dc.subject | PATHWAY | - |
dc.subject | ANGIOGENESIS | - |
dc.subject | EXPRESSION | - |
dc.subject | GENE | - |
dc.subject | IDENTIFICATION | - |
dc.subject | PROLIFERATION | - |
dc.title | CXXC5 is a transcriptional activator of Flk-1 and mediates bone morphogenic protein-induced endothelial cell differentiation and vessel formation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Suhyun | - |
dc.contributor.affiliatedAuthor | Park, Hae-Chul | - |
dc.identifier.doi | 10.1096/fj.13-236216 | - |
dc.identifier.scopusid | 2-s2.0-84897089336 | - |
dc.identifier.wosid | 000331072200010 | - |
dc.identifier.bibliographicCitation | FASEB JOURNAL, v.28, no.2, pp.615 - 626 | - |
dc.relation.isPartOf | FASEB JOURNAL | - |
dc.citation.title | FASEB JOURNAL | - |
dc.citation.volume | 28 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 615 | - |
dc.citation.endPage | 626 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | EMBRYONIC STEM-CELLS | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | BINDING DOMAIN | - |
dc.subject.keywordPlus | VEGF | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordAuthor | HUVECs | - |
dc.subject.keywordAuthor | mouse embryonic stem cells | - |
dc.subject.keywordAuthor | caudal vein plex vessel formation | - |
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