First Thermostable Endo-beta-1,4-Glucanase from Newly Isolated Xanthomonas sp EC102
DC Field | Value | Language |
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dc.contributor.author | Woo, Mi-Hee | - |
dc.contributor.author | Chang, Young-Hyo | - |
dc.contributor.author | Lee, Hoi-Seon | - |
dc.contributor.author | Pak, Pyo June | - |
dc.contributor.author | Kim, Joong-Su | - |
dc.contributor.author | Chung, Namhyun | - |
dc.date.accessioned | 2021-09-05T12:00:08Z | - |
dc.date.available | 2021-09-05T12:00:08Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-02 | - |
dc.identifier.issn | 1572-3887 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/99485 | - |
dc.description.abstract | A novel gene encoding thermostable endoglucanase was identified in Xanthomonas sp. EC102 from soil. The gene had 1,458 base pairs of open reading frame, which encode a 52-kDa protein of 486 amino acid residues. Sequence of the amino acid residues was similar with the endoglucanase from Xanthomonas campestris pv. campestris ATCC33913 (GenBank Accession No. NP_638867.1) (94 % identity). The endoglucanase was overexpressed in Escherichia coli BL21 and purified. Temperature for the highest enzymatic activity was 70 A degrees C and pH optima was pH 5.5. The specific activity of the endoglucanase toward carboxymethylcellulose (CMC) was approximately 2 mu mol min(-1) mg(-1), V (max) for CMC was 1.44 mu mol mg(-1) min(-1), and K (m) values was 25.6 mg mL(-1). The EC102 endoglucanase was stable at temperatures up to 60 A degrees C, and it was activated by 0.1 mM of Mn2+ and Co2+. This is the first report about thermostable endoglucanase from Xanthomonas sp. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | ISOLATED BACILLUS-SUBTILIS | - |
dc.subject | CELLULASE | - |
dc.subject | EXPRESSION | - |
dc.subject | ENZYMES | - |
dc.subject | GENE | - |
dc.subject | CLONING | - |
dc.title | First Thermostable Endo-beta-1,4-Glucanase from Newly Isolated Xanthomonas sp EC102 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Namhyun | - |
dc.identifier.doi | 10.1007/s10930-013-9535-9 | - |
dc.identifier.scopusid | 2-s2.0-84898596717 | - |
dc.identifier.wosid | 000330176500012 | - |
dc.identifier.bibliographicCitation | PROTEIN JOURNAL, v.33, no.1, pp.110 - 117 | - |
dc.relation.isPartOf | PROTEIN JOURNAL | - |
dc.citation.title | PROTEIN JOURNAL | - |
dc.citation.volume | 33 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 110 | - |
dc.citation.endPage | 117 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | ISOLATED BACILLUS-SUBTILIS | - |
dc.subject.keywordPlus | CELLULASE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordAuthor | Thermostability | - |
dc.subject.keywordAuthor | Xanthomonas sp. | - |
dc.subject.keywordAuthor | Endoglucanase | - |
dc.subject.keywordAuthor | Characterization | - |
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