Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Osteogenesis induction of periodontal ligament cells onto bone morphogenic protein-2 immobilized PCL fibers

Authors
Kim, Sung EunYun, Young-PilHan, Yun-KyungLee, Deok-WonOhe, Joo-YoungLee, Baek-SooSong, Hae-RyongPark, KyeongsoonChoi, Byung-Joon
Issue Date
2-1월-2014
Publisher
ELSEVIER SCI LTD
Keywords
Periodontal ligament cells (PDLCs); Bone morphogenic protein-2 (BMP-2); PCL fiber; Osteogenesis induction
Citation
CARBOHYDRATE POLYMERS, v.99, pp.700 - 709
Indexed
SCIE
SCOPUS
Journal Title
CARBOHYDRATE POLYMERS
Volume
99
Start Page
700
End Page
709
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/99561
DOI
10.1016/j.carbpol.2013.08.053
ISSN
0144-8617
Abstract
The purpose of this study was to develop bone morphogenic protein-2 (BMP-2) immobilized PCL fibers to induce osteogenic differentiation of periodontal ligament cells (PDLCs). The PCL fiber surface was modified with heparin-dopamine (Hep-DOPA) (Hep-PCL) and further immobilized with BMP-2 (BMP-2/Hep-PCL). PCL fibers and surface-modified PCL fibers (Hep-PCL and BMP-2/Hep-PCL) were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle. Osteogenic differentiation of PDLCs was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and gene expression. The results of XPS and contact angle revealed that Hep-DOPA and BMP-2 were successfully immobilized onto the PCL surface and that the BMP-2/Hep-PCL fibers have more hydrophilic surface properties than PCL fibers alone. ALP activity, calcium deposition, and gene expression on BMP-2/Hep-PCL fibers showed significantly induced osteogenic differentiation relative to PCL fibers. Therefore, we suggest that BMP-2/Hep-PCL fibers have the potential to effectively induce osteogenic differentiation of PDLCs. (C) 2013 Published by Elsevier Ltd.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biomedical Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE