Demethylation of RUNX3 by Vincristine in Colorectal Adenocarcinoma Cells
DC Field | Value | Language |
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dc.contributor.author | Moon, Ji Wook | - |
dc.contributor.author | Lee, Soo Kyung | - |
dc.contributor.author | Lee, Jung Ok | - |
dc.contributor.author | Kim, Ji Hae | - |
dc.contributor.author | Kim, Nami | - |
dc.contributor.author | Kim, Jin | - |
dc.contributor.author | Kim, Hyeon Soo | - |
dc.contributor.author | Park, Sun-Hwa | - |
dc.date.accessioned | 2021-09-05T12:36:07Z | - |
dc.date.available | 2021-09-05T12:36:07Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-01 | - |
dc.identifier.issn | 0250-7005 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/99625 | - |
dc.description.abstract | Background: Methylation-mediated inactivation of tumor-suppressor genes is a critical event during the pathogenesis of many malignancies. Vincristine is a conventional anticancer drug used to treat various types of cancers. However, few studies describe the epigenetic-based effects of vincristine. In this study, changes in the methylation of runt-related transcription factor-3 (RUNX3) were investigated in CCD18Co normal colon cells and DLD-1 colorectal adenocarcinoma cells. Materials and Methods: CCD18Co and DLD-1 cells were treated with vincristine, and the methylation status was assessed using quantitative methylation-specific polymerase chain reaction (QMSP). Eleven normal colon tissues and 105 colorectal cancer tissues were investigated by methylation and mRNA expression of RUNX3 using QMSP and real-time reverse transcription polymerase chain reaction (real time-PCR). Results: RUNX3 was demethylated after vincristine treatment in DLD-1 cells. The expression of RUNX3 mRNA was down-regulated in DLD-1 cells because of DNA hypermethylation, but was restored after vincristine treatment. In addition, hypermethylation of RUNX3 was detected in 70 out of 105 colorectal carcinomas (66.7%). RUNX3 hypermethylation was greater in colon cancer tissues than in rectal cancer tissues. The expression of RUNX3 mRNA was reduced in 68 out of 105 colorectal cancer tissues (64.8%). Conclusion: These results demonstrate that vincristine demethylates RUNX3 in colorectal adenocarcinoma cells, and restores its expression. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | INT INST ANTICANCER RESEARCH | - |
dc.subject | TUMOR-SUPPRESSOR GENES | - |
dc.subject | PROMOTER HYPERMETHYLATION | - |
dc.subject | COMBINATION CHEMOTHERAPY | - |
dc.subject | EPIGENETIC INACTIVATION | - |
dc.subject | DNA HYPERMETHYLATION | - |
dc.subject | CANCER-PATIENTS | - |
dc.subject | CYCLOPHOSPHAMIDE | - |
dc.subject | METHYLATION | - |
dc.subject | THERAPY | - |
dc.subject | TUMORIGENESIS | - |
dc.title | Demethylation of RUNX3 by Vincristine in Colorectal Adenocarcinoma Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jin | - |
dc.contributor.affiliatedAuthor | Kim, Hyeon Soo | - |
dc.contributor.affiliatedAuthor | Park, Sun-Hwa | - |
dc.identifier.scopusid | 2-s2.0-84897027174 | - |
dc.identifier.wosid | 000329765300014 | - |
dc.identifier.bibliographicCitation | ANTICANCER RESEARCH, v.34, no.1A, pp.133 - 140 | - |
dc.relation.isPartOf | ANTICANCER RESEARCH | - |
dc.citation.title | ANTICANCER RESEARCH | - |
dc.citation.volume | 34 | - |
dc.citation.number | 1A | - |
dc.citation.startPage | 133 | - |
dc.citation.endPage | 140 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | TUMOR-SUPPRESSOR GENES | - |
dc.subject.keywordPlus | PROMOTER HYPERMETHYLATION | - |
dc.subject.keywordPlus | COMBINATION CHEMOTHERAPY | - |
dc.subject.keywordPlus | EPIGENETIC INACTIVATION | - |
dc.subject.keywordPlus | DNA HYPERMETHYLATION | - |
dc.subject.keywordPlus | CANCER-PATIENTS | - |
dc.subject.keywordPlus | CYCLOPHOSPHAMIDE | - |
dc.subject.keywordPlus | METHYLATION | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | TUMORIGENESIS | - |
dc.subject.keywordAuthor | DNA methylation | - |
dc.subject.keywordAuthor | vincristine | - |
dc.subject.keywordAuthor | colonic neoplasms | - |
dc.subject.keywordAuthor | demethylation | - |
dc.subject.keywordAuthor | methylation-specific polymerase chain reaction | - |
dc.subject.keywordAuthor | RUNX3 | - |
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