Metformin Reduces TGF-beta 1-Induced Extracellular Matrix Production in Nasal Polyp-Derived Fibroblasts
- Authors
- Park, Il-Ho; Um, Ji-Young; Hong, Sung-Moon; Cho, Jung-Sun; Lee, Seung Hoon; Lee, Sang Hag; Lee, Heung-Man
- Issue Date
- 1월-2014
- Publisher
- SAGE PUBLICATIONS LTD
- Keywords
- nasal polyposis; metformin; adenosine monophosphate-activated protein kinase; fibroblast; fibronectin
- Citation
- OTOLARYNGOLOGY-HEAD AND NECK SURGERY, v.150, no.1, pp.148 - 153
- Indexed
- SCIE
SCOPUS
- Journal Title
- OTOLARYNGOLOGY-HEAD AND NECK SURGERY
- Volume
- 150
- Number
- 1
- Start Page
- 148
- End Page
- 153
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/99653
- DOI
- 10.1177/0194599813513880
- ISSN
- 0194-5998
- Abstract
- Background and Objects Metformin is widely used to treat type 2 diabetes mellitus, and adenosine monophosphate-activated protein kinase (AMPK) is thought to be the target that mediates its effects. Recently, it has been demonstrated that metformin has antifibrotic effects beyond its antihyperglycemic action. The purposes of this study were to investigate the effect of metformin on TGF-1-induced myofibroblast differentiation (-smooth muscle actin [-SMA]) and extracellular matrix (ECM) production and to determine the underlying mechanism of the action of metformin in nasal polyp-derived fibroblasts (NPDFs). Study Design Basic research. Setting The rhinology laboratory of Korea University Guro Hospital, Seoul, Korea. Methods NPDFs from 7 patients were incubated with TGF-1 and treated with metformin or compound C, an inhibitor of AMPK. To determine the proliferation rate of nasal fibroblasts, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was performed. The expression levels of -SMA and fibronectin were determined by reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and immunofluorescent staining. Phosphorylation of AMPK and phosphorylation of Smad2/3 were evaluated by Western blot analysis. Results In TGF-1-induced NPDFs, metformin inhibited the expression of -SMA and fibronectin, as confirmed by both RT-PCR and Western blot analysis. Metformin increased the phosphorylation of AMPK and the expression levels of -SMA and fibronectin. However, compound C reversed these effects. Metformin inhibited TGF-1-induced phosphorylation of Smad2/3. Conclusions This study showed that metformin inhibits TGF-1-induced myofibroblast differentiation and ECM production in NPDFs via the Smad2/3 pathway. AMPK can be a therapeutic target for the prevention of ECM remodeling in nasal polyps.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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