High-temperature GC-MS-based serum cholesterol signatures may reveal sex differences in vasospastic angina
- Authors
- Son, Hyun-Hwa; Moon, Ju-Yeon; Seo, Hong Seog; Kim, Hyun Hee; Chung, Bong Chul; Choi, Man Ho
- Issue Date
- 1월-2014
- Publisher
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- Keywords
- hybrid solid-phase extraction-precipitation; cardiovascular disease; hydroxycholesterol
- Citation
- JOURNAL OF LIPID RESEARCH, v.55, no.1, pp.155 - 162
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF LIPID RESEARCH
- Volume
- 55
- Number
- 1
- Start Page
- 155
- End Page
- 162
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/99692
- DOI
- 10.1194/jlr.D040790
- ISSN
- 0022-2275
- Abstract
- Alterations of cholesterol metabolism are responsible for vasospastic angina and atherosclerosis. To comprehensively evaluate cholesterol metabolism, 18 sterols, including cholesterol, 6 cholesteryl esters (CEs), 3 cholesterol precursors, and 8 hydroxycholesterols (OHCs), were simultaneously analyzed using hybrid solid-phase extraction (SPE) purification coupled to high-temperature gas chromatography-mass spectrometry (HTGC-MS). Methanol-based hybrid SPE increased the selective extraction, and HTGC resulted in a good chromatographic resolution for the separation of lipophilic compounds. The limits of quantification of cholesterol and CEs ranged from 0.2 to 10.0 mu g/ml, while OHCs and cholesterol precursors ranged from 0.01 to 0.10 mu g/ml. Linearity as the correlation coefficient was higher than 0.99 with the exception of cholesteryl laurate, myristate, oleate, and linoleate (r(2) > 0.98). The precision (%coefficient of variation) and accuracy (%bias) ranged from 1.1 to 9.8% and from 75.9 to 125.1%, respectively. The overall recoveries of CEs ranged from 26.1 to 64.0%, and the recoveries of other sterols ranged from 83.8 to 129.3%. The cholesterol signatures showed sex differences in patients with vasospastic angina and may associate with 24-reductases. This technique can be useful for making clinical diagnoses and for an increased understanding of the pathophysiology of vasospastic angina.
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