Effects of sFlt-1 and alpha 2-macroglobulin on vascular endothelial growth factor-induced endothelin-1 upregulation in human microvascular endothelial cells
DC Field | Value | Language |
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dc.contributor.author | Yi, K. W. | - |
dc.contributor.author | Jung, S. H. | - |
dc.contributor.author | Cho, G. J. | - |
dc.contributor.author | Seol, H. J. | - |
dc.contributor.author | Hong, S. C. | - |
dc.contributor.author | Oh, M. J. | - |
dc.contributor.author | Kim, H. J. | - |
dc.date.accessioned | 2021-09-05T12:48:54Z | - |
dc.date.available | 2021-09-05T12:48:54Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-01 | - |
dc.identifier.issn | 0143-4004 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/99708 | - |
dc.description.abstract | Introduction: Soluble fms-like tyrosine kinase-1 (sFlt-1) is a vascular endothelial growth factor (VEGF) binding protein and potent antagonist of VEGF. Alpha 2 macroglobulin (alpha M-2) is another major binding protein for circulating VEGF, which is present in human plasma at higher concentration (2-4 mg/mL) than sFlt-1. This study investigated the effects of sFlt-1 and alpha M-2 on VEGF-induced endothelin-1 (ET-1) upregulation in human microvascular endothelial cell-1 (HMEC-1). Methods: HMEC-1 was cultured and incubated with varying concentrations of sFlt-1 and alpha M-2 in combination with VEGF. ET-1 mRNA expression in the cells was measured by real time RT-PCR and ET-1 protein by western blot analysis. Results: ET-1 expression in HMEC-1 incubated with VEGF significantly increased in time- and dose-dependent manners. Next, HMEC-1 was treated with the sFlt-1 (10-1000 ng/mL) or alpha M-2 (10-10000 ng/mL) in the presence of VEGF (10 ng/mL). We found that sFlt-1 induced a significant decrease of ET-1 expression upregulated by VEGF, while alpha M-2 did not affect the VEGF-induced ET-1 expression. Conclusions: sFLT-1 suppressed the VEGF-induced the ET-1 expression of HMEC-1. However, alpha M-2 did not show a significant effect on the ET-1 expression that was induced by VEGF. The results suggest that a certain proportion of the bound form alpha M-2-VEGF have a biological action involved in the pathophysiology of preeclampsia. (C) 2013 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | W B SAUNDERS CO LTD | - |
dc.subject | NORMAL PREGNANCIES | - |
dc.subject | TYROSINE KINASE-1 | - |
dc.subject | RANDOMIZED-TRIAL | - |
dc.subject | PREECLAMPSIA | - |
dc.subject | PLASMA | - |
dc.subject | BINDING | - |
dc.subject | EXPRESSION | - |
dc.subject | RECEPTORS | - |
dc.subject | WOMEN | - |
dc.subject | HYPERTENSION | - |
dc.title | Effects of sFlt-1 and alpha 2-macroglobulin on vascular endothelial growth factor-induced endothelin-1 upregulation in human microvascular endothelial cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, M. J. | - |
dc.identifier.doi | 10.1016/j.placenta.2013.09.008 | - |
dc.identifier.scopusid | 2-s2.0-84896542289 | - |
dc.identifier.wosid | 000330916500009 | - |
dc.identifier.bibliographicCitation | PLACENTA, v.35, no.1, pp.64 - 69 | - |
dc.relation.isPartOf | PLACENTA | - |
dc.citation.title | PLACENTA | - |
dc.citation.volume | 35 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 64 | - |
dc.citation.endPage | 69 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Developmental Biology | - |
dc.relation.journalResearchArea | Obstetrics & Gynecology | - |
dc.relation.journalResearchArea | Reproductive Biology | - |
dc.relation.journalWebOfScienceCategory | Developmental Biology | - |
dc.relation.journalWebOfScienceCategory | Obstetrics & Gynecology | - |
dc.relation.journalWebOfScienceCategory | Reproductive Biology | - |
dc.subject.keywordPlus | NORMAL PREGNANCIES | - |
dc.subject.keywordPlus | TYROSINE KINASE-1 | - |
dc.subject.keywordPlus | RANDOMIZED-TRIAL | - |
dc.subject.keywordPlus | PREECLAMPSIA | - |
dc.subject.keywordPlus | PLASMA | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | WOMEN | - |
dc.subject.keywordPlus | HYPERTENSION | - |
dc.subject.keywordAuthor | alpha(2)-macroglobulin | - |
dc.subject.keywordAuthor | sFlt-1 | - |
dc.subject.keywordAuthor | Endothelin-1 | - |
dc.subject.keywordAuthor | Preeclampsia | - |
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