Identification of antioxidant constituents of the aerial part of Plantago asiatica using LC-MS/MS coupled DPPH assay

Ahn, Jong Hoon; Jo, Yang Hee; Kim, Seon Beom; Turk, Ayman; Oh, Kyung-Eon; Hwang, Bang Yeon; Lee, Ki Yong; Lee, Mi Kyeong

doi:10.1016/j.phytol.2018.05.006

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Identification of antioxidant constituents of the aerial part of Plantago asiatica using LC-MS/MS coupled DPPH assay

Ahn, Jong Hoon;
Jo, Yang Hee;
Kim, Seon Beom;
Turk, Ayman;
Oh, Kyung-Eon;
... Lee, Ki Yong;
외 2명

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15

초록

Screening of EtOAc soluble fraction of Plantago asiatica (Plantaginaceae) using HPLC-MS/MS coupled DPPH online assay suggested the presence of antioxidant compounds, which characterized as phenylethanoid glycosides. Twenty-nine compounds including three new compounds, plantalides A-C, a phenylethanoid (1), a phenolic compound (9) and an iridoid (10), were isolated from P. asiatica. Consistent with HPLC-MS/MS analysis, seven phenylethanoid glycosides (1-7) including a new phenylethanoid glycoside (1) showed antioxidant with ranging IC50 values of 22.9-88.5 mu M. These results suggested that phenylethanoid glycosides were contributed to the antioxidant activity of P. asiatica and HPLC-MS/MS coupled online assay is powerful tool for predicting active compounds with short time and limited amounts.

키워드

Plantago asiatica; Plantaginaceae; phenylethanoid; LC-MS/MS online assay; Iridoid; OXIDATIVE STRESS; GLYCOSIDES

제목: Identification of antioxidant constituents of the aerial part of Plantago asiatica using LC-MS/MS coupled DPPH assay

저자: Ahn, Jong Hoon; Jo, Yang Hee; Kim, Seon Beom; Turk, Ayman; Oh, Kyung-Eon; Hwang, Bang Yeon; Lee, Ki Yong; Lee, Mi Kyeong

DOI: 10.1016/j.phytol.2018.05.006

발행일: 2018-08

유형: Article

저널명: Phytochemistry Letters

권: 26

페이지: 20 ~ 24