Identification of antioxidant constituents of the aerial part of Plantago asiatica using LC-MS/MS coupled DPPH assay

  • Ahn, Jong Hoon; 
  • Jo, Yang Hee; 
  • Kim, Seon Beom; 
  • Turk, Ayman; 
  • Oh, Kyung-Eon; 
  • ... Lee, Ki Yong; 
  • 외 2명
Citations

WEB OF SCIENCE

12
Citations

SCOPUS

15

초록

Screening of EtOAc soluble fraction of Plantago asiatica (Plantaginaceae) using HPLC-MS/MS coupled DPPH online assay suggested the presence of antioxidant compounds, which characterized as phenylethanoid glycosides. Twenty-nine compounds including three new compounds, plantalides A-C, a phenylethanoid (1), a phenolic compound (9) and an iridoid (10), were isolated from P. asiatica. Consistent with HPLC-MS/MS analysis, seven phenylethanoid glycosides (1-7) including a new phenylethanoid glycoside (1) showed antioxidant with ranging IC50 values of 22.9-88.5 mu M. These results suggested that phenylethanoid glycosides were contributed to the antioxidant activity of P. asiatica and HPLC-MS/MS coupled online assay is powerful tool for predicting active compounds with short time and limited amounts.

키워드

Plantago asiatica; Plantaginaceae; phenylethanoid; LC-MS/MS online assay; Iridoid; OXIDATIVE STRESS; GLYCOSIDES
제목
Identification of antioxidant constituents of the aerial part of Plantago asiatica using LC-MS/MS coupled DPPH assay
저자
Ahn, Jong Hoon; Jo, Yang Hee; Kim, Seon Beom; Turk, Ayman; Oh, Kyung-Eon; Hwang, Bang Yeon; Lee, Ki Yong; Lee, Mi Kyeong
DOI
10.1016/j.phytol.2018.05.006
발행일
2018-08
유형
Article
저널명
Phytochemistry Letters
권
26
페이지
20 ~ 24