Production of 2?-fucosyllactose using a1,2-fucosyltransferase from a GRAS bacterial strain

초록

2 '-Fucosyllactose (2 '-FL) is a major oligosaccharide found in human breast milk. It is produced from GDP-L-fucose and D-lactose by a1,2-fucosyltransferase (a1,2-fucT), but the enzyme has been identified mostly in pathogens. In this study, an a1,2-fucT was isolated from a Generally Recognized as Safe (GRAS) Bacillus mega-terium strain. The enzyme was successfully expressed in metabolically-engineered Escherichia coli. Furthermore, replacement of non-conserved amino acid residues with conserved ones in the protein led to an increase in the rate of 2 '-FL production. As a result, fed-batch fermentation of E. coli produced 30 g/L of 2 '-FL from glucose and lactose. Thus, the overproduction of 2 '-FL using a novel enzyme from a GRAS bacteria strain was successfully demonstrated.

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