Rheinheimera faecalis sp. nov., isolated from Ceratotherium simum feces

초록

A novel strain YR1(T), Gram-stain-negative, rod-shaped, catalase- and oxidase-positive, and aerobic bacterium, was isolated from the feces of Ceratotherium simum. The strain grew at 9-42 degrees C (optimal temperature, 30 degrees C), at pH 6.0-10.0 (optimal pH, 7.0), and in the presence of 0-3% (w/v) NaCl (optimal salinity, 0%). Phylogenetic analyses based on 16S rRNA gene sequencing indicated that strain YR1(T) was most closely related to Rheinheimera soli BD-d46(T) (98.6%), R. riviphila KYPC3(T) (98.6%), and R. mangrovi LHK 132(T) (98.1%). Moreover, the average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization values between strain YR1(T) and R. mangrovi LHK 132( T) were 88.3%, 92.1%, and 35.3%, respectively, indicating that strain YR1(T) is a novel species in the genus Rheinheimera. The genome size and genomic DNA G + C content of strain YR1(T) were 4.5 Mbp and 46.37%, respectively. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol, while the predominant respiratory quinone was Q-8. Summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), C-16: 0, and summed feature 8 (C-18:1 omega 7c) were the primary cellular fatty acids (> 16%). Based on these genotypic and phenotypic characteristics, strain YR1(T) was identified as a novel species in the genus Rheinheimera, for which the name Rheinheimera faecalis sp. nov. is proposed, with the type strain is YR1(T) (= KACC 22402(T) = JCM 34823(T)).

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