A novel helper qPCR system for platinum detection via Pt-DNA coordination

Cai, Sheng; Yang, Dan; Tian, Xueke; Ye, Jiawei; Xu, Mingcheng; Al-Maskri, Abdu Ahmed Abdullah; Jung, Cheulhee; Zeng, Su

doi:10.1016/j.aca.2018.11.007

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A novel helper qPCR system for platinum detection via Pt-DNA coordination

Cai, Sheng;
Yang, Dan;
Tian, Xueke;
Ye, Jiawei;
Xu, Mingcheng;
... Jung, Cheulhee;
외 2명

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초록

A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qPCR, was introduced in our helper qPCR system. Several guanine (G) bases were introduced in the helper template to obtain a platinum-responsive on/off switch based on G-Pt-G coordination. Because of the helper template, a slight change in platinum concentration would significantly change the signal in the qPCR. This novel helper qPCR technique easily detects platinum with high sensitivity (1 ng/mL) and selectivity over other metal ions. Therefore, it will be a promising technique for the detection of platinum in biomedical and environmental samples. (c) 2018 Elsevier B.V. All rights reserved.

키워드

Helper qPCR platform; Pt-DNA coordination; Platinum-responsive on/off switch; Platinum detection; ELECTROCHEMICAL BIOSENSOR; LIQUID-CHROMATOGRAPHY; SENSITIVE DETECTION; INTACT CISPLATIN; BLOOD-PLASMA; HUMAN SERUM; ICP-MS; OXALIPLATIN; IONS; MECHANISMS

제목: A novel helper qPCR system for platinum detection via Pt-DNA coordination

저자: Cai, Sheng; Yang, Dan; Tian, Xueke; Ye, Jiawei; Xu, Mingcheng; Al-Maskri, Abdu Ahmed Abdullah; Jung, Cheulhee; Zeng, Su

DOI: 10.1016/j.aca.2018.11.007

발행일: 2019-03-07

유형: Article

저널명: Analytica Chimica Acta

권: 1050

페이지: 154 ~ 160