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초록
The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis. (c) 2008 Elsevier B.V. All rights reserved.
키워드
hMSCs; Percoll; plasma membrane; two-phase extraction; two-dimensional electrophoresis; Ultracentrifugation; GEL-ELECTROPHORESIS; 2-PHASE PARTITION; PROTEINS; EXTRACTION; SEPARATION; CELLS
- 제목
- Sample preparation method for plasma membrane proteome analysis
- 저자
- Park, Seung-Ah; Kim, Mi-Ryung; Kim, Pan-Kyeom; Cho, Hyung-Don; Han, Gi-Yeon; Kim, Chan-Wha
- 발행일
- 2008-09-01
- 유형
- Article
- 권
- 872
- 호
- 1-2
- 페이지
- 177 ~ 180