Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles

Lee, Yeon-Ji; Park, Jong-Moon; Huh, Jiyoung; Kim, Minsik; Lee, Je-Sun; Palani, Arudra; Lee, Kwangyeol; Lee, Sang-Won

doi:10.5012/bkcs.2010.31.6.1568

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Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles

Lee, Yeon-Ji;
Park, Jong-Moon;
Huh, Jiyoung;
Kim, Minsik;
Lee, Je-Sun;
... Lee, Kwangyeol;
... Lee, Sang-Won;
외 1명

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6

초록

The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathione-modified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/MS/MS experiments.

키워드

Proteomics; Glutathione; Superparamagnetic; Nanoparticle; Separation; TRANSFERASE FUSION PROTEINS; MASS-SPECTROMETRY; SELECTIVE ENRICHMENT; MAGNETIC NANOPARTICLES; PHOSPHORYLATED PEPTIDES; QUANTIFICATION; IDENTIFICATION; PURIFICATION; PROBE; SIRNA

제목: Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles

저자: Lee, Yeon-Ji; Park, Jong-Moon; Huh, Jiyoung; Kim, Minsik; Lee, Je-Sun; Palani, Arudra; Lee, Kwangyeol; Lee, Sang-Won

DOI: 10.5012/bkcs.2010.31.6.1568

발행일: 2010-06-20

유형: Article

저널명: Bulletin of the Korean Chemical Society

권: 31

호: 6

페이지: 1568 ~ 1572