Kaistella rhinocerotis sp. nov., isolated from the faeces of rhinoceros and reclassification of Chryseobacterium faecale as Kaistella faecalis comb. nov.

초록

Strain Ran72 T , a novel Gram- stain- negative, obligately aerobic, non- motile, and rod- shaped bacterium, was isolated from the faeces of the rhinoceros species Ceratotherium simum . The novel bacterial strain grew optimally in Reasoner's 2A medium under the following conditions: 0 % (w/v) NaCl, pH 7.5, and 30 degrees C. Based on phylogenetic analysis using 16S rRNA gene sequencing, strain Ran72 T was found to be most closely related to Chryseobacterium faecale F4 T (98.4 %), Kaistella soli DKR-2 T (98.0 %), and Kaistella haifensis H38 T (97.4 %). A comprehensive genome- level comparison between strain Ran72 T with C. faecale F4 T , K. soli DKR-2 T , and K. haifensis H38 T revealed average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values of <= 74.9, <= 19.3, and <= 78.7 %, respectively. The major fatty acids were anteiso- C 15: 0 (22.3 %), with MK - 6 being the predominant respiratory quinone. The major polar lipids of strain Ran72 T were phosphatidylethanolamine, four unidentified aminolipids, and two unidentified lipids. Based on our chemotaxonomic, genotypic, and phenotype characterizations, strain Ran72 T was identified as representing a novel species in the genus Kaistella , for which the name Kaistella rhinocerotis sp. nov. is proposed, with the type strain Ran72 T (=KACC 23136 T =JCM 36038 T ). Based on the outcomes of our phylogenomic study, Chryseobacterium faecale should be reclassified under the genus Kaistella as Kaistella faecalis comb. nov.

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