Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

Ryu, Seung Mok; Kwon, Jaeyoung; Seo, Young Hye; Song, Eun Gyeong; Hong, Seong Su; Kim, Beom Seok; Hong, Jin Sung; Ryu, Ki Hyun; Lee, Dongho

doi:10.1016/j.virusres.2016.09.013

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Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

Ryu, Seung Mok;
Kwon, Jaeyoung;
Seo, Young Hye;
Song, Eun Gyeong;
Hong, Seong Su;
... Kim, Beom Seok;
... Lee, Dongho;
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초록

A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (1), bruceantin (2), brucein A (3), bruceantinol (4), and brucein B (5), from the CH3OH extract of Brucea javanica. All isolated compounds exhibited inactivation effects in systemic host plants, and compounds 3 and 4 were potent, with a minimum inhibitory concentration of 10 M. Furthermore, compound 3 was found to have a protective effect at the tested concentration of 40 M. (C) 2016 Elsevier B.V. All rights reserved.

키워드

Pepper mottle virus; Green fluorescent protein; Capsicum annuum; Brucea javanica; Quassinoid; Antiviral agent; MOSAIC-VIRUS; STRUCTURAL ELUCIDATION; AGENTS; SEEDS

제목: Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

저자: Ryu, Seung Mok; Kwon, Jaeyoung; Seo, Young Hye; Song, Eun Gyeong; Hong, Seong Su; Kim, Beom Seok; Hong, Jin Sung; Ryu, Ki Hyun; Lee, Dongho

DOI: 10.1016/j.virusres.2016.09.013

발행일: 2017-01-02

유형: Article

저널명: Virus Research

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