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초록
We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn2+](m)). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn2+ with a dissociation constant (K-d(TP)) of 3.1 +/- 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn2+](m) in a rat hippocampal slice at a depth of 100-200 mu m without interference from other metal ions through the use of two-photon microscopy.
키워드
FLUORESCENT-PROBES; METAL-IONS; ZN2+; ALZHEIMERS; DISEASE; SENSORS; TISSUE; CELLS
- 제목
- A Mitochondrial-Targeted Two-Photon Probe for Zinc Ion
- 저자
- Masanta, Goutam; Lim, Chang Su; Kim, Hyung Joong; Han, Ji Hee; Kim, Hwan Myung; Cho, Bong Rae
- 발행일
- 2011-04-20
- 유형
- Article
- 권
- 133
- 호
- 15
- 페이지
- 5698 ~ 5700