Inhibition of Diacylglycerol Acyltransferase by Phenylpyropenes Produced by Penicillium griseofulvum F1959

초록

Phenylpyropenes A, B, and C, isolated from Penicillium griseofulvum F1959, inhibited DGAT in rat liver microsomes with IC50 values of 78.7 +/- 1.6, 21.7 +/- 0.2, and 11.04 +/- 0.2 mu M, respectively. In addition, a kinetic analysis using a Lineweaver-Burk plot revealed that phenylpyropene C was a noncompetitive inhibitor of DGAT. The apparent Michaelis constant (K-m) value and inhibition constant (K-i) value were calculated to be 8 mu M and 10.4 mu M, respectively. Moreover, phenylpyropene C inhibited triglyceride formation in HepG2 cells.

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