Dual-Color Imaging of Magnesium/Calcium Ion Activities with Two-Photon Fluorescent Probes

  • Dong, Xiaohu
  • Han, Ji Hee
  • Heo, Cheol Ho
  • Kim, Hwan Myung
  • Liu, Zhihong
  • 외 1명
Citations

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초록

We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg2+ ([Mg2+](i)) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca2+ ([Ca2+](m)), FMg2 allows dual-color imaging of Mg2+/Ca2+ activities in live cells and [Mg2+](i)/[Ca2+](m) distributions in live tissues at a depth of 100-200 mu m.

키워드

INTRACELLULAR MAGNESIUMBINDING
제목
Dual-Color Imaging of Magnesium/Calcium Ion Activities with Two-Photon Fluorescent Probes
저자
Dong, XiaohuHan, Ji HeeHeo, Cheol HoKim, Hwan MyungLiu, ZhihongCho, Bong Rae
DOI
10.1021/ac302210v
발행일
2012-10-02
유형
Article
저널명
Analytical Chemistry
84
19
페이지
8110 ~ 8113