Chryseobacterium cheonjiensesp. nov., isolated from forest soil

초록

A yellow-pigmented, non-motile and rod-shaped bacterium, designated RJ-7-14(T)was obtained from forest soil sampled at Cheonji-dong, Seogwipo-si, Jeju-do, South Korea. Cells were Gram-stain-negative and produced flexirubin type pigments. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RJ-7-14(T)formed a lineage within the familyWeeksellaceaeand clustered as members of the genusChryseobacterium. The closest members wereChryseobacterium geocarposphaeraeDSM 27617(T)(98.2% sequence similarity),Chryseobacterium hispalenseDSM 25574(T)(98.0%) andChryseobacterium nepalenseKACC 18907(T)(98.0%). The sequence similarity for other members was < 98.0%. The genome was 4,276,416 bp long with 9 scaffolds and 3779 protein-coding genes. The sole respiratory quinone was MK-6. The major cellular fatty acids were iso-C-15:0, summed feature 9 (iso-C-17:1 omega 9cand/or C(16:0)10-methyl), summed feature 3 (iso-C(15:0)2-OH and/or C-16: 1 omega 7c) and iso-C(17:0)3-OH. The major polar lipid was phosphatidylethanolamine (PE). The DNA G + C content of the type strain was 37.2 mol%. In addition, the average nucleotide identity (ANIu) and in silico DNA-DNA hybridization (dDDH) relatedness values between strain RJ-7-14(T)and phylogenetically closest members were <= 88.2% and <= 35.0%, respectively, which were below the threshold values of 95-96% (for ANI) and 70% (for dDDH), suggesting the allocation of novel strain to a new species. Based on genomic, chemotaxonomic, phenotypic and phylogenetic analyses, strain RJ-7-14(T)represents novel species in the genusChryseobacterium, for which the nameChryseobacterium cheonjiensesp. nov. is proposed. The type strain is RJ-7-14(T)(= KACC 21625(T) = NBRC 114362(T)).

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