Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture

Shin, Hyun Yong; Lee, Jin Young; Park, Chulhwan; Kim, Seung Wook

doi:10.1016/j.jbiotec.2010.12.021

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Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture

Shin, Hyun Yong;
Lee, Jin Young;
Park, Chulhwan;
Kim, Seung Wook

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5

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6

초록

In this study, the role of glycerol in cephalosporin C production was evaluated in Acremonium chrysogenum M35. Methionine-unsupplemented culture with glycerol stimulated cephalosporin C production in A. chrysogenum M35 by up to 10-fold while increasing the amount of cysteine. Glycerol caused the upregulation of isopenicillin N-CoA epimerase (cefD2) and delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthase (pcbAB) transcription in exponential phase. These results indicate that glycerol without methionine effectively enhanced cephalosporin C production via stimulation of cysteine and valine in A. chrysogemun M35. (C) 2011 Elsevier B.V. All rights reserved.

키워드

Cysteine; Acremonium chrysogenum; Methionine; Cephalosporin C; CYSTATHIONINE-GAMMA-LYASE; PENICILLIN-N; ISOPENICILLIN-N; FATTY-ACIDS; MECB GENE; BIOSYNTHESIS; VALINE; STIMULATION; SYNTHETASE; CONVERSION

제목: Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture

저자: Shin, Hyun Yong; Lee, Jin Young; Park, Chulhwan; Kim, Seung Wook

DOI: 10.1016/j.jbiotec.2010.12.021

발행일: 2011-02-20

유형: Article

저널명: Journal of Biotechnology

권: 151

호: 4

페이지: 363 ~ 368