Stimulation of cephalosporin C production in Acremonium chrysogenum M35 by glycerol

Shin, Hyun Yong; Lee, Jin Young; Jung, You Ree; Kim, Seung Wook

doi:10.1016/j.biortech.2010.01.095

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Stimulation of cephalosporin C production in Acremonium chrysogenum M35 by glycerol

Shin, Hyun Yong;
Lee, Jin Young;
Jung, You Ree;
Kim, Seung Wook

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초록

In this study, the effects of glycerol on cephalosporin C production by Acremonium chrysogenum M35 were evaluated. The addition of glycerol increased cephalosporin production by up to 12-fold. Glycerol caused the upregulation of the transcription of the isopenicillin synthase (pcbC) and transporter (cefT) genes in early exponential phase, and affected the cell morphology since hyphal fragments differentiated into arthrospores. These results indicate that glycerol effectively enhances cephalosporin C production via stimulation of cell differentiation. (C) 2010 Elsevier Ltd. All rights reserved.

키워드

Acremonium chrysogenum; Isopenicillin N synthase; Transporter; Glycerol; Morphological characteristics; TRANSCRIPTION FACTOR CPCR1; CLAVULANIC ACID PRODUCTION; CYSTATHIONINE-GAMMA-LYASE; BETA-LACTAM BIOSYNTHESIS; STREPTOMYCES-CLAVULIGERUS; CARBON SOURCE; CEFT GENE; MECB GENE; OPTIMIZATION; ENHANCEMENT

제목: Stimulation of cephalosporin C production in Acremonium chrysogenum M35 by glycerol

저자: Shin, Hyun Yong; Lee, Jin Young; Jung, You Ree; Kim, Seung Wook

DOI: 10.1016/j.biortech.2010.01.095

발행일: 2010-06

유형: Article

저널명: Bioresource Technology

권: 101

호: 12

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