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Asiatic acid induces apoptosis and autophagy and reduces MiR-17 and MiR-21 expression in pancreatic cancer cell lines
- Jo, Y.-G.;
- Kim, M.;
- Shin, H.;
- Lee, K.Y.;
- Lee, E.J.
SCOPUS
0초록
− This study investigated the cytotoxic effects and mechanism of action of asiatic acid in pancreatic cancer cell lines. First, we confirmed the cell viability of MIA PaCa-2 and PANC-1 cells after asiatic acid administration for 48 and 72 h. The viability of MIA PaCa-2 and PANC-1 cells decreased in a dose-dependent manner following asiatic acid administration. To investigate the underlying mechanism, we performed a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, annexin V assay, and western blotting. Asiatic acid induced apoptosis and autophagy through activation of AMP-activated protein kinase (AMPK) and inhibition of mammalian target of rapamycin (mTOR) in MIA PaCa-2 cells. Finally, the expression of miR-17 and miR-21, known as oncogenes in pancreatic cancer, was decreased by asiatic acid. These results indicate that asiatic acid has potential as a new therapeutic agent against pancreatic cancer. © 2019, Korean Society of Pharmacognosy. All rights reserved.
키워드
- 제목
- Asiatic acid induces apoptosis and autophagy and reduces MiR-17 and MiR-21 expression in pancreatic cancer cell lines
- 저자
- Jo, Y.-G.; Kim, M.; Shin, H.; Lee, K.Y.; Lee, E.J.
- 발행일
- 2019
- 유형
- Article
- 권
- 25
- 호
- 4
- 페이지
- 298 ~ 303