Cold-shock gene cspC in the genome of Massilia polaris sp. nov. revealed cold-adaptation

초록

A straw coloured, motile and Gram-stain-negative bacterium, designated RP-1-19(T) was isolated from soil of Arctic station, Svalbard, Norway. Based on the phylogenetic analysis of its 16S rRNA gene sequence, strain RP-1-19(T) formed a lineage within the family Oxalobacteraceae and clustered together within the genus Massilia. The closest members were M. violaceinigra B2(T) (98.6% sequence similarity), M. eurypsychrophilia JCM 30074(T) (98.3%) and M. atriviolacea SODT (98.1%). The only respiratory quinone was ubiquinone-8. The principal cellular fatty acids were summed feature 3 (iso-C-15:0 2-OH/C-16:1 omega 7c) and C-16:0. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G + C content of the type strain was 63.2%. The average nucleotide identity and in silico DNA-DNA hybridization values between strain RP-1-19(T) and closest members were <= 80 and 23.2%, respectively. The genome was 4,522,469 bp long with 30 scaffolds and 4076 protein-coding genes. The genome showed eight putative biosynthetic gene clusters responsible for various secondary metabolites. Genome analysis revealed the presence of cold-shock proteins CspA and CspC. Presence of cspA and cspC genes in the genome manifest ecophysiology of strain RP-1-19(T) that may help in cold-adaptation. Based on these data, strain RP-1-19(T) represents a novel species in the genus Massilia, for which the name Massilia polaris sp. nov. is proposed. The type strain is RP-1-19(T) (= KACC 21619(T) = NBRC 114359(T)).

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