Chryseobacterium antibioticum sp. nov. with antimicrobial activity against Gram-negative bacteria, isolated from Arctic soil

초록

A yellow-pigmented, non-motile, Gram-stain-negative, pleomorphic bacterium, designated RP-3-3(T) was obtained from soil sampled at the Arctic region in Cambridge Bay, NU, Canada. The strain is strictly aerobic, psychrotolerant, grow optimally at 15-20 degrees C and produces flexirubin type pigments. The strain is able to hydrolyse CM-cellulose, casein, starch and DNA. Strain RP-3-3(T) showed antimicrobial activity against Gram-negative pathogens. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RP-3-3(T) formed a lineage within the family Weeksellaceae and clustered as members of the genus Chryseobacterium. The closest members were Chryseobacterium shigense DSM 17126(T) (98.7% sequence similarity),Chryseobacterium carnipullorum DSM 25581(T) (98.7%) and Chryseobacterium oncorhychi701B-08(T)(98%). The genome is 4,910,468 bp long with 73 scaffolds and 4300 protein-coding genes. The anti-SMASH analysis of whole genome showed ten putative biosynthetic gene clusters responsible for various secondary metabolites. The sole respiratory quinone is MK-6. The major cellular fatty acids are iso-C-15:0, iso-C-17:1 omega 9c, iso-C(17:0)3-OH, summed feature 3 (iso-C(15 :0)2-OH/C-16 :1 omega 7c) and anteiso-C-15:0. The major polar lipid is phosphatidylethanolamine. The DNA G + C content of the type strain is 36.9 mol%. In addition, the average nucleotide identity andin silicoDNA-DNA hybridization relatedness values between strain RP-3-3(T) and phylogenetically closest members are below the threshold value for species delineation. Based on genomic, chemotaxonomic, phenotypic and phylogenetic analyses, strain RP-3-3(T) represents novel species in the genusChryseobacterium, for which the name Chryseobacterium antibioticumsp. nov. is proposed. The type strain is RP-3-3(T)(=KACC 21620(T) = NBRC 114360(T)).

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