The Potential of Exosomes Derived from Chronic Myelogenous Leukaemia Cells as a Biomarker

Kang, Ka-Won; Jung, Jik-Han; Hur, Woojune; Park, Jaena; Shin, Hyunku; Choi, Byeonghyeon; Jeong, Hyesun; Kim, Dae Sik; Yu, Eun Sang; Lee, Se Ryeon; Sung, Hwa Jung; Kim, Seok Jin; Choi, Chul Won; Kim, Hyun Koo; Hong, Sunghoi; Park, Ji-Ho; Choi, Yeonho; Park, Yong; Kim, Byung Soo

doi:10.21873/anticanres.12679

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The Potential of Exosomes Derived from Chronic Myelogenous Leukaemia Cells as a Biomarker

Kang, Ka-Won;
Jung, Jik-Han;
Hur, Woojune;
Park, Jaena;
Shin, Hyunku;
... Kim, Dae Sik;
... Lee, Se Ryeon;
... Sung, Hwa Jung;
... Choi, Chul Won;
... Kim, Hyun Koo;
... Hong, Sunghoi;
... Choi, Yeonho;
... Park, Yong;
... Kim, Byung Soo;
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초록

Background/Aim: Exosomes, derived from chronic myelogenous leukaemia (CML) cells, can be used as biomarkers and new targets for the detection of the BCR-ABL transcript. This study aimed to identify these possibilities. Materials and Methods: Human CML cell line-derived exosomes and CML-patients-derived exosomes were isolated with a size-exclusion chromatography column and ExoQuick (TM) exosome precipitation solution, respectively. Isolated exosomes were analysed by nested PCR to detect the BCR-ABL transcript. Results: Exosomes derived from the two human CML cell lines yielded a 250-bp band. RNA sequence analysis revealed 99% sequence homology with the partial mRNA for the human BCR-ABL chimeric protein. This similar to 250-bp band was also observed in the exosomes derived from patients with CML. However, only patients at the blast and accelerated phases showed the exosomal BCR-ABL transcript. Conclusion: CML-derived exosomes could act as novel targets for the detection of the BCR-ABL transcript.

키워드

Chronic myelogenous leukaemia; exosome; BCR-ABL; biomarker; CHRONIC MYELOID-LEUKEMIA; ACUTE LYMPHOBLASTIC-LEUKEMIA; BCR-ABL TRANSCRIPTS; BONE-MARROW; EXTRACELLULAR VESICLES; TRANSPLANTATION; CANCER; RECOMMENDATIONS; INHIBITORS; DISEASE

제목: The Potential of Exosomes Derived from Chronic Myelogenous Leukaemia Cells as a Biomarker

저자: Kang, Ka-Won; Jung, Jik-Han; Hur, Woojune; Park, Jaena; Shin, Hyunku; Choi, Byeonghyeon; Jeong, Hyesun; Kim, Dae Sik; Yu, Eun Sang; Lee, Se Ryeon; Sung, Hwa Jung; Kim, Seok Jin; Choi, Chul Won; Kim, Hyun Koo; Hong, Sunghoi; Park, Ji-Ho; Choi, Yeonho; Park, Yong; Kim, Byung Soo

DOI: 10.21873/anticanres.12679

발행일: 2018-07

유형: Article

저널명: Anticancer Research

권: 38

호: 7

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