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초록
We report a two-photon (TP) probe (ACaLN) for near-membrane Ca2+ that shows a 13-fold TP excited fluorescence (TPEF) enhancement in response to Ca2+, dissociation constants (K-d(TP)) of (1.9 +/- 0.2) mu m, pH-insensitivity at the biologically relevant pH, and can detect near-membrane Ca2+ in live cells for more than 1500 s and in living tissues at 120 mu m depth without interference from other metal ions. Comparison with existing probes provides a useful strategy for the design of efficient TP probes for the near-membrane metal ions.
키워드
calcium; fluorescence; nonlinear spectroscopy; microscopy; two-photon probe; CA2+; INDICATORS; CELLS
- 제목
- In Vivo Imaging of Near-Membrane Calcium Ions with Two-Photon Probes
- 저자
- Lim, Chang Su; Kang, Min Young; Han, Ji Hee; Danish, Isravel Antony; Cho, Bong Rae
- 발행일
- 2011-08-01
- 유형
- Article
- 권
- 6
- 호
- 8
- 페이지
- 2027 ~ 2032