Caenimonas soli sp. nov., isolated from soil

초록

A non-motile, Gram-stain-negative, rod-shaped bacterium, designated strain S4(T), was obtained from soil sampled at Wonju, Gyeonggi-do, Republic of Korea. Cells were white-coloured, aerobic, grew optimally at 25-32 degrees C on R2A agar plate. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain S4(T) formed a lineage within the family Comamonadaceae. The closest members were Caenimonas terrae SGM1-15(T) (98.1% sequence similarity), Caenimonas koreensis EMB320(T) (97.5%) and Ramlibacter solisilvae 5-10(T) (97.8%). The sequence similarities of strain S4(T) with other members of the family Comamonadaceae were <= 97.5%. The sole respiratory quinone was ubiquinone-8 (Q-8) and the principal polar lipid was phosphatidylethanolamine. The predominant cellular fatty acids were summed feature 3 (iso-C-15 :0 2-OH/C-16 :1 omega 7c), C-16:0 and summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c). The DNA G + C content was 65.1 mol%. In addition, the average nucleotide identity (ANIu) and in silico DNA-DNA hybridization (dDDH) relatedness values between strain S4(T) and Caenimonas koreensis were 77.6 and 21%, respectively. Based on genomic, chemotaxonomic, phenotypic, and phylogenetic analyses, strain S4(T) represents a novel species in the genus Caenimonas, for which the name Caenimonas soli sp. nov. is proposed. The type strain is S4(T) (= KCTC 72742(T) = NBRC 114610(T)).

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