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Cd2+ binds to Atx1 and affects the physical interaction between Atx1 and Ccc2 in Saccharomyces cerevisiae
- Heo, Dong-Hyuk;
- Baek, In-Joon;
- Kang, Hyun-Jun;
- Kim, Ji-Hyun;
- Chang, Miwha;
- ... Yun, Cheol-Won;
- 외 1명
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12초록
The ATX1 deletion strain of Saccharomyces cerevisiae is more resistant to Cd2+ than the wild-type. To investigate the function of Atx1 in Cd2+ toxicity, we used a metal-binding assay to study the interaction between Atx1 and Cd2+ in vitro. Using circular dichroism and two-hybrid analyses, we found that Atx1 can bind Cd2+ specifically and that Cd2+ binding to Atx1 affects the physical interaction between Atx1 and Ccc2. These results imply that Atx1 delivers Cd2+ to Ccc2 and that this delivery is, at least in part, responsible for Cd2+ toxicity in S. cerevisiae.
키워드
Atx1; Cadmium; Ccc2; Copper; Saccharomyces cerevisiae; COPPER TRANSPORT; OXYGEN-TOXICITY; IRON TRANSPORT; CADMIUM; GENE; PROTEIN; HOMEOSTASIS; CHAPERONE
- 제목
- Cd2+ binds to Atx1 and affects the physical interaction between Atx1 and Ccc2 in Saccharomyces cerevisiae
- 저자
- Heo, Dong-Hyuk; Baek, In-Joon; Kang, Hyun-Jun; Kim, Ji-Hyun; Chang, Miwha; Kang, Chang-Min; Yun, Cheol-Won
- 발행일
- 2012-02
- 유형
- Article
- 권
- 34
- 호
- 2
- 페이지
- 303 ~ 307
- 언어
- ENG
- 출판사
- SPRINGER
- 발행국가
- 네덜란드
- 분량
- 5 페이지
- ISSN
- E 1573-6776
P 0141-5492