Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS

  • Lien, Truong Thi Ngoc
  • Lam, Tran Dai
  • An, Vu Thi Hong
  • Hoang, Tran Vinh
  • Quang, Duong Tuan
  • ... Kim, Jong Seung
  • 외 3명
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초록

In this paper, we describe DNA electrochemical detection for genetically modified organism (GMO) based on multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole (PPy). DNA hybridization is studied by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). An increase in DNA complementary target concentration results in a decrease in the faradic charge transfer resistance (R-ct) and signifying "signal-on" behavior of MWCNTs-PPy-DNA system. QCM and EIS data indicated that the electroanalytical MWCNTs-PPy films were highly sensitive (as low as 4 pM of target can be detected with QCM technique). In principle, this system can be suitable not only for DNA but also for protein biosensor construction. (C) 2009 Elsevier B.V. All rights reserved.

키워드

MWCNTs-doped polypyrroleElectrochemical DNA biosensorQuartz crystal microbalanceElectrochemical impedance spectroscopyCONDUCTING POLYMERSOLIGONUCLEOTIDESSENSORSFILM
제목
Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS
저자
Lien, Truong Thi NgocLam, Tran DaiAn, Vu Thi HongHoang, Tran VinhQuang, Duong TuanKhieu, Dinh QuangTsukahara, ToshifumiLee, Young HoonKim, Jong Seung
DOI
10.1016/j.talanta.2009.09.002
발행일
2010-01-15
유형
Article
저널명
Talanta
80
3
페이지
1164 ~ 1169