<i>Jannaschia ovalis</i> sp. nov., a marine bacterium isolated from a tidal flat sediment in the Republic of Korea

초록

A Gram-stain-negative, non-motile, aerobic, pale-yellow coloured and oval-shaped bacterium designated GRR-S6-38(T) was isolated from a tidal flat sediment that collected from Garorim Bay of the Yellow Sea, Republic of Korea. Strain GRR-S6-38(T) grew at 15-40 degrees C (optimum, 30 degrees C) at pH 6-9 (optimum, pH 7) and at 2-6% (w/v) NaCl (optimum, 2% NaCl). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain GRR-S6-38(T) was closely related to those of J. seosinensis CL-SP26(T) (98.1%), J. helgolandensis DSM 14858T (97.4%), J. rubra CECT 5088(T) (97.4%), J. faecimaris DSM 100420T (97.4%), J. marina SHC163(T) (97.3%), J. donghaensis CECT 7802(T) (97.2%) and J. pohangensis DSM 19073(T) (97.1%). The average nucleotide identity and digital DNA-DNA hybridization value between GRR-S6-38T and related type strains were 71.47-78.59% and 17.80-21.40%. Strain GRR-S6-38(T) was characterized as having Q-10 as the predominant respiratory quinone and the major principle fatty acids (> 10%) were SF 8 (C18:1 omega 7c/C18:1 omega 6c, 39.3%), C19:0 omega 8c cyclo (13.2%) and C18:1 omega 7c 11-methyl (10.6%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified lipid, two unidentified aminolipids and two unidentified phospholipids. The assembled genome of strain GRR-S6-38(T) has a total length of 3.32 Mbp, and a G + C content is 69.98%. Based on the polyphasic taxonomic evidence presented in this study, GRR-S6-38T is regarded to represent a novel species within the genus Jannaschia, for which name Jannaschia ovalis sp. nov. is proposed. The type strain is GRR-S6-38(T) (= KCTC 82518 = KACC 22240 = JCM 36187).

키워드