Expression, purification, crystallization and preliminary X-ray analysis of the human NORE1 SARAH domain

  • Kim, Hye Jin
  • Hwang, Eunha
  • Han, Young-Hyun
  • Choi, Saehae
  • Lee, Woo Cheol
  • ... Jeon, Young Ho
  • 외 3명
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초록

NORE1 is an important tumour suppressor in human cancers that interacts with the pro-apoptotic protein kinase MST1/2 through SARAH domains. The SARAH domain (residues 366-413) of human NORE1 was expressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to 2.7 angstrom resolution and belonged to space group P6(1)22, with unit-cell parameters a = b = 73.041, c = 66.092 angstrom, alpha = beta = 90, gamma = 120 degrees.

키워드

apoptosisNORE1Rastumour suppressorsTUMOR-SUPPRESSORIDENTIFICATIONPROTEINSBINDINGPATHWAY
제목
Expression, purification, crystallization and preliminary X-ray analysis of the human NORE1 SARAH domain
저자
Kim, Hye JinHwang, EunhaHan, Young-HyunChoi, SaehaeLee, Woo CheolKim, Hye-YeonJeon, Young HoCheong, ChaejoonCheong, Hae-Kap
DOI
10.1107/S1744309112021744
발행일
2012-07
유형
Article
저널명
Acta Crystallographica Section F: Structural Biology Communications
68
페이지
813 ~ 815