Tocopheryl oligochitosan-based self assembling oligomersomes for siRNA delivery

초록

Amphiphilic alpha-tocopherol oligochitosan conjugates were constructed by conjugating alpha-tocopherol succinate to water soluble oligochitosans with various molecular weights In aqueous medium the tocopherol oligochitosan conjugates self-assembled to single layered oligomersomes The sizes of alpha-tocopherol-oligochitosan-based oligomersomes (TCOsomes) could be controlled by chain lengths of oligochitosans The mean sizes of TCOsomes were 220 and 377 nm as the sizes of oligochitosans were 4000 and 12 500 respectively For all TCOsomes formed in this study polydispersity indexes were in the ranges of 0 111-0 256 Cryo-TEM images showed clear thickening in the unilamellar layer of TCOsomes upon complexation with siRNAs Zeta potentials decreased as the ratios of siRNA/TCOsomes increased TCOsomes self-assembled from tocopherol-oligochitosan 4K (TCOsome(4K)) significantly enhanced the cellular uptake of siRNAs (>98%) and reduced the expression of target proteins more effectively than did Lipofectamine 2000 In tumor xenografted mice the intratumoral administration of siMcl-1 using TCOsomes substantially silenced the expression of Mcl-1 and prevented the growth of tumor The hematoxylin-eosin staining showed the apoptosis of cells in the tissues of the mice treated with siMcl-1/TCOsome(4K) complexes but not with siGL2/TCOsome(4K) complexes The self-assembling and size-controllable oligomersomes might be suitable for effective in vivo delivery of siRNAs (C) 2010 Elsevier Ltd All rights reserved

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