Dual-functional fluorescent molecular rotor for endoplasmic reticulum microviscosity imaging during reticulophagy

He, Ying; Shin, Jinwoo; Gong, Wanjun; Das, Pintu; Qu, Jinghan; Yang, Zhigang; Liu, Wufan; Kang, Chulhun; Qu, Junle; Kim, Jong Seung

doi:10.1039/c9cc00300b

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Dual-functional fluorescent molecular rotor for endoplasmic reticulum microviscosity imaging during reticulophagy

He, Ying;
Shin, Jinwoo;
Gong, Wanjun;
Das, Pintu;
Qu, Jinghan;
... Kim, Jong Seung;
외 4명

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71

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71

초록

The microviscosity change associated with reticulophagy is an important component for studying endoplasmic reticulum (ER) stress disorders. Here, a BODIPY-arsenicate conjugate 1-based fluorescent molecular rotor was designed to covalently bind vicinal dithiol-containing proteins in the ER, exhibiting a bifunction of reticulophagy initiation and microviscosity evaluation. Therefore, we could quantify the local viscosity changes during reticulophagy based on the fluorescence lifetime changes of probe 1.

키워드

DITHIOL-CONTAINING PROTEINS; ER STRESS; ARSENIC-BINDING; VISCOSITY; AUTOPHAGY; PROBE; LC3B; CELL; MITOCHONDRIA; TARGET

제목: Dual-functional fluorescent molecular rotor for endoplasmic reticulum microviscosity imaging during reticulophagy

저자: He, Ying; Shin, Jinwoo; Gong, Wanjun; Das, Pintu; Qu, Jinghan; Yang, Zhigang; Liu, Wufan; Kang, Chulhun; Qu, Junle; Kim, Jong Seung

DOI: 10.1039/c9cc00300b

발행일: 2019-02-28

유형: Article

저널명: Chemical Communications

권: 55

호: 17

페이지: 2453 ~ 2456